Detailed information NTG0591935

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KZ795_RS07780
• Genome accession: NZ_CP080286
• Coordinates: 1695225..1696373 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1690225..1701373

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZ795_RS07755 (KZ795_07755)	-	1691376..1691672 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KZ795_RS07760 (KZ795_07760)	-	1691669..1692262 (-)	594	WP_003084544.1	YggT family protein	-
KZ795_RS07765 (KZ795_07765)	proC	1692273..1693094 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
KZ795_RS07770 (KZ795_07770)	-	1693106..1693798 (-)	693	WP_033972290.1	YggS family pyridoxal phosphate-dependent enzyme	-
KZ795_RS07775 (KZ795_07775)	pilT	1694013..1695047 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KZ795_RS07780 (KZ795_07780)	pilU	1695225..1696373 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KZ795_RS07785 (KZ795_07785)	czcD	1696381..1697280 (-)	900	WP_024918014.1	CDF family zinc transporter CzcD	-
KZ795_RS07790 (KZ795_07790)	-	1697434..1697838 (+)	405	WP_003084560.1	NINE protein	-
KZ795_RS07795 (KZ795_07795)	-	1698081..1699454 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
KZ795_RS07800 (KZ795_07800)	-	1699451..1700635 (+)	1185	WP_033972291.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=591935 KZ795_RS07780 WP_003100959.1 1695225..1696373(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF12]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=591935 KZ795_RS07780 WP_003100959.1 1695225..1696373(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF12]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGTTGTCGCCGGAACAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTTTTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCCGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382