Detailed information NTG0591901

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KZ799_RS08735
• Genome accession: NZ_CP080281
• Coordinates: 1911573..1912721 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1906573..1917721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZ799_RS08710 (KZ799_08710)	-	1907724..1908020 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KZ799_RS08715 (KZ799_08715)	-	1908017..1908610 (-)	594	WP_003084544.1	YggT family protein	-
KZ799_RS08720 (KZ799_08720)	proC	1908621..1909442 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
KZ799_RS08725 (KZ799_08725)	-	1909454..1910146 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KZ799_RS08730 (KZ799_08730)	pilT	1910361..1911395 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KZ799_RS08735 (KZ799_08735)	pilU	1911573..1912721 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KZ799_RS08740 (KZ799_08740)	czcD	1912729..1913628 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KZ799_RS08745 (KZ799_08745)	-	1913782..1914186 (+)	405	WP_003084560.1	NINE protein	-
KZ799_RS08750 (KZ799_08750)	-	1914429..1915802 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
KZ799_RS08755 (KZ799_08755)	-	1915799..1916983 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=591901 KZ799_RS08735 WP_003100959.1 1911573..1912721(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF17]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=591901 KZ799_RS08735 WP_003100959.1 1911573..1912721(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF17]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382