Detailed information NTG0591881

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KZ794_RS28065
• Genome accession: NZ_CP080280
• Coordinates: 6076032..6077180 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6071032..6082180

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZ794_RS28045 (KZ794_28045)	-	6071769..6072953 (-)	1185	WP_016852373.1	cystathionine gamma-synthase	-
KZ794_RS28050 (KZ794_28050)	-	6072950..6074323 (-)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
KZ794_RS28055 (KZ794_28055)	-	6074566..6074970 (-)	405	WP_003084560.1	NINE protein	-
KZ794_RS28060 (KZ794_28060)	czcD	6075125..6076024 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KZ794_RS28065 (KZ794_28065)	pilU	6076032..6077180 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KZ794_RS28070 (KZ794_28070)	pilT	6077358..6078392 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KZ794_RS28075 (KZ794_28075)	-	6078607..6079299 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KZ794_RS28080 (KZ794_28080)	proC	6079311..6080132 (+)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
KZ794_RS28085 (KZ794_28085)	-	6080143..6080736 (+)	594	WP_003084544.1	YggT family protein	-
KZ794_RS28090 (KZ794_28090)	-	6080733..6081029 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=591881 KZ794_RS28065 WP_003100959.1 6076032..6077180(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF20]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=591881 KZ794_RS28065 WP_003100959.1 6076032..6077180(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF20]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382