Detailed information NTG0590749

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KZH79_RS19170
• Genome accession: NZ_CP080011
• Coordinates: 4214148..4215296 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain TL3773
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4209148..4220296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZH79_RS19145 (KZH79_19145)	-	4210299..4210595 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KZH79_RS19150 (KZH79_19150)	-	4210592..4211185 (-)	594	WP_003084544.1	YggT family protein	-
KZH79_RS19155 (KZH79_19155)	proC	4211196..4212017 (-)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
KZH79_RS19160 (KZH79_19160)	-	4212029..4212721 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KZH79_RS19165 (KZH79_19165)	pilT	4212936..4213970 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KZH79_RS19170 (KZH79_19170)	pilU	4214148..4215296 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KZH79_RS19175 (KZH79_19175)	czcD	4215304..4216203 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KZH79_RS19180 (KZH79_19180)	-	4216358..4216762 (+)	405	WP_003084560.1	NINE protein	-
KZH79_RS19185 (KZH79_19185)	-	4217005..4218378 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
KZH79_RS19190 (KZH79_19190)	-	4218375..4219559 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=590749 KZH79_RS19170 WP_003100959.1 4214148..4215296(+) (pilU) [Pseudomonas aeruginosa strain TL3773]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=590749 KZH79_RS19170 WP_003100959.1 4214148..4215296(+) (pilU) [Pseudomonas aeruginosa strain TL3773]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382