Detailed information NTG0589372

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M1S39_RS14465
• Genome accession: NZ_CP096738
• Coordinates: 2993445..2994563 (+)
• Length: 372 a.a.
• NCBI ID: WP_249379572.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 5679
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2988445..2999563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1S39_RS14455 (M1S39_14455)	-	2991561..2992253 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
M1S39_RS14460 (M1S39_14460)	pilT	2992380..2993417 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
M1S39_RS14465 (M1S39_14465)	pilU	2993445..2994563 (+)	1119	WP_249379572.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M1S39_RS14470 (M1S39_14470)	fur	2994633..2995070 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
M1S39_RS14475 (M1S39_14475)	bamE	2995182..2995580 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
M1S39_RS14480 (M1S39_14480)	-	2995635..2995959 (-)	325	Protein_2827	RnfH family protein	-
M1S39_RS14485 (M1S39_14485)	-	2995959..2997026 (-)	1068	WP_002019572.1	hypothetical protein	-
M1S39_RS14490 (M1S39_14490)	-	2997179..2997634 (-)	456	WP_000782976.1	bacteriohemerythrin	-
M1S39_RS14495 (M1S39_14495)	-	2997887..2998735 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41920.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=589372 M1S39_RS14465 WP_249379572.1 2993445..2994563(+) (pilU) [Acinetobacter baumannii strain 5679]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKTSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=589372 M1S39_RS14465 WP_249379572.1 2993445..2994563(+) (pilU) [Acinetobacter baumannii strain 5679]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAACCAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	76.19	100	0.774
pilU	Pseudomonas stutzeri DSM 10701	69.837	98.925	0.691
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395