Detailed information NTG0587950

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M1S18_RS14555
• Genome accession: NZ_CP096693
• Coordinates: 2982730..2983848 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 5773
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2977730..2988848

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1S18_RS14545 (M1S18_14545)	-	2980846..2981538 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
M1S18_RS14550 (M1S18_14550)	pilT	2981665..2982702 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
M1S18_RS14555 (M1S18_14555)	pilU	2982730..2983848 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M1S18_RS14560 (M1S18_14560)	fur	2983918..2984355 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
M1S18_RS14565 (M1S18_14565)	bamE	2984467..2984865 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
M1S18_RS14570 (M1S18_14570)	-	2984920..2985243 (-)	324	WP_000442587.1	RnfH family protein	-
M1S18_RS14575 (M1S18_14575)	-	2985243..2986310 (-)	1068	WP_002019572.1	hypothetical protein	-
M1S18_RS14580 (M1S18_14580)	-	2986463..2986918 (-)	456	WP_000782976.1	bacteriohemerythrin	-
M1S18_RS14585 (M1S18_14585)	-	2987171..2988019 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=587950 M1S18_RS14555 WP_000347039.1 2982730..2983848(+) (pilU) [Acinetobacter baumannii strain 5773]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=587950 M1S18_RS14555 WP_000347039.1 2982730..2983848(+) (pilU) [Acinetobacter baumannii strain 5773]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395