NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	KW114_RS04150	Genome accession	NZ_CP079097
Coordinates	944128..944994 (-)	Length	288 a.a.
NCBI ID	WP_218798023.1	Uniprot ID	-
Organism	Methylococcus capsulatus strain KN2
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 939128..949994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KW114_RS04115 (KW114_04115)	-	939277..939852 (-)	576	WP_010961329.1	RNA pyrophosphohydrolase	-
KW114_RS04120 (KW114_04120)	-	940040..940702 (+)	663	WP_017366374.1	HAD family phosphatase	-
KW114_RS04125 (KW114_04125)	-	940711..940941 (-)	231	WP_010961331.1	sulfurtransferase TusA family protein	-
KW114_RS04130 (KW114_04130)	cysG	940990..942414 (+)	1425	WP_218798019.1	siroheme synthase CysG	-
KW114_RS04135 (KW114_04135)	yacG	942427..942642 (-)	216	WP_218798020.1	DNA gyrase inhibitor YacG	-
KW114_RS04140 (KW114_04140)	zapD	942655..943368 (-)	714	WP_371131307.1	cell division protein ZapD	-
KW114_RS04145 (KW114_04145)	coaE	943524..944120 (-)	597	WP_218798022.1	dephospho-CoA kinase	-
KW114_RS04150 (KW114_04150)	pilD	944128..944994 (-)	867	WP_218798023.1	A24 family peptidase	Machinery gene
KW114_RS04155 (KW114_04155)	pilC	945018..946232 (-)	1215	WP_010961338.1	type II secretion system F family protein	Machinery gene
KW114_RS04160 (KW114_04160)	pilB	946237..947952 (-)	1716	WP_010961339.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

Download Length: 288 a.a. Molecular weight: 31038.62 Da Isoelectric Point: 7.2397

                                >NTDB_id=587667 KW114_RS04150 WP_218798023.1 944128..944994(-) (pilD) [Methylococcus capsulatus strain KN2]

MLLPTGPAGVALCGSFGLIIGSFLNVVIHRLPLMMEQAWRRECGELLEPGGGRPAGETEYNLWRPGSQCPHCRAPVRFWQ

NVPVFSYLWLRGRCAACGTAISWRYPLVETLTGVLFAMVAWHFGASWQALAACALTAGLIALAFIDLDHLVLPDDITLPL

LWLGLLLNAQGLFCSLETALYGAVAGYLLLWTVHRGFLLVTGREGMGFGDFKLLAMLGAWMGWPMLPVIILFSSISGAVI

GSIALAVGGKGRDTPIPFGPFLAAGGWIALLWGNALNDAYWRWAAPGG

Nucleotide

Download Length: 867 bp

                                >NTDB_id=587667 KW114_RS04150 WP_218798023.1 944128..944994(-) (pilD) [Methylococcus capsulatus strain KN2]

ATGTTGTTGCCGACCGGGCCGGCGGGGGTTGCCCTCTGCGGATCGTTCGGACTGATCATCGGGAGTTTTCTCAACGTGGT

CATCCACCGCCTGCCGCTCATGATGGAGCAGGCCTGGCGCAGGGAGTGCGGGGAGTTGCTCGAGCCCGGCGGCGGACGGC

CGGCGGGGGAGACCGAATACAACCTGTGGCGACCCGGATCGCAGTGTCCGCACTGCCGGGCGCCGGTCCGGTTCTGGCAG

AATGTCCCCGTGTTCAGCTATCTTTGGCTGCGTGGCCGTTGTGCCGCTTGCGGCACGGCGATTTCCTGGCGTTATCCCCT

GGTCGAAACGCTCACGGGGGTGCTGTTCGCAATGGTGGCTTGGCATTTCGGCGCGTCCTGGCAGGCACTGGCGGCGTGCG

CCCTAACGGCTGGCCTGATCGCCCTGGCTTTCATCGACCTGGATCACCTGGTACTGCCGGACGACATCACGCTTCCCCTG

TTGTGGCTGGGGCTGCTGCTGAACGCCCAAGGTCTCTTCTGCAGTCTGGAGACGGCGCTGTACGGTGCCGTTGCAGGCTA

TCTCCTGCTATGGACGGTGCATCGCGGCTTTCTGCTCGTGACCGGCCGGGAGGGCATGGGGTTCGGCGACTTCAAGCTAC

TCGCCATGCTCGGTGCCTGGATGGGTTGGCCGATGCTGCCGGTCATCATCCTGTTCTCATCCATCTCGGGTGCTGTGATC

GGTTCGATTGCGCTGGCAGTCGGCGGCAAGGGGAGGGACACGCCGATTCCCTTCGGGCCGTTTCTGGCGGCGGGCGGCTG

GATCGCCCTGCTGTGGGGAAATGCGCTCAACGACGCCTATTGGCGCTGGGCCGCTCCTGGAGGTTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(134-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(16-123)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Neisseria gonorrhoeae MS11	50.542	96.181	0.486
pilD	Vibrio cholerae strain A1552	50.735	94.444	0.479
pilD	Vibrio campbellii strain DS40M4	48.718	94.792	0.462
pilD	Acinetobacter baumannii D1279779	49.057	92.014	0.451
pilD	Acinetobacter nosocomialis M2	48.679	92.014	0.448

Detailed information NTG0587667