Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KW114_RS04160 Genome accession   NZ_CP079097
Coordinates   946237..947952 (-) Length   571 a.a.
NCBI ID   WP_010961339.1    Uniprot ID   Q606C2
Organism   Methylococcus capsulatus strain KN2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 941237..952952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW114_RS04135 (KW114_04135) yacG 942427..942642 (-) 216 WP_218798020.1 DNA gyrase inhibitor YacG -
  KW114_RS04140 (KW114_04140) zapD 942655..943368 (-) 714 WP_371131307.1 cell division protein ZapD -
  KW114_RS04145 (KW114_04145) coaE 943524..944120 (-) 597 WP_218798022.1 dephospho-CoA kinase -
  KW114_RS04150 (KW114_04150) pilD 944128..944994 (-) 867 WP_218798023.1 A24 family peptidase Machinery gene
  KW114_RS04155 (KW114_04155) pilC 945018..946232 (-) 1215 WP_010961338.1 type II secretion system F family protein Machinery gene
  KW114_RS04160 (KW114_04160) pilB 946237..947952 (-) 1716 WP_010961339.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KW114_RS04165 (KW114_04165) - 948002..950767 (-) 2766 WP_218798024.1 valine--tRNA ligase -
  KW114_RS04170 (KW114_04170) - 950835..951293 (-) 459 WP_218798025.1 DNA polymerase III subunit chi -
  KW114_RS04175 (KW114_04175) - 951290..952783 (-) 1494 WP_218798026.1 leucyl aminopeptidase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63100.82 Da        Isoelectric Point: 5.5480

>NTDB_id=587669 KW114_RS04160 WP_010961339.1 946237..947952(-) (pilB) [Methylococcus capsulatus strain KN2]
MAAVSGVQQLSGLAKSLVKAQLLSEADAQAYFEDARRKNTPFVSYLVANKILDGLQIAQVAHQEFGLPLLDLDAVVFDIP
PTQWVGEKLIRRHHILPLLRRGNHLFVAVSDPTNFLGLDEITFQTRLVTDCVLVEENKLSRCIEAVLDAADTSLKQLLEE
DLDSLQITAGDDEGPHAEPEISGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPPALGVK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPSSAQIGVEMLGFEPEQRDNFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVEANISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCPRCKREEKVPPEVLLA
SGFREEDIGNFTLFGPAGCDQCVKGYKGRVGIYQVMPISEEINRIILEGGNVMALTEQAHAEGIADLRESGLKKVKAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=587669 KW114_RS04160 WP_010961339.1 946237..947952(-) (pilB) [Methylococcus capsulatus strain KN2]
ATGGCGGCGGTTTCCGGCGTCCAGCAGCTGAGCGGTCTGGCGAAAAGCCTGGTCAAGGCCCAGTTGCTTTCGGAGGCGGA
CGCCCAGGCGTACTTCGAGGATGCGCGCAGGAAGAACACCCCTTTCGTCAGCTATCTCGTCGCCAACAAGATCCTGGACG
GTCTCCAGATCGCCCAGGTCGCCCACCAGGAGTTCGGGCTGCCCCTGCTCGATCTGGACGCAGTCGTCTTCGACATTCCC
CCCACGCAGTGGGTCGGCGAGAAGCTGATACGCCGCCATCACATCCTGCCCTTGCTGCGACGGGGGAATCACCTCTTCGT
CGCGGTCTCGGATCCGACCAATTTCCTGGGACTGGATGAAATCACTTTCCAGACCCGTCTCGTCACCGACTGTGTCCTGG
TCGAGGAGAACAAGCTTTCGCGCTGCATCGAGGCGGTCCTGGATGCGGCGGATACCAGCCTGAAGCAGCTGCTCGAAGAG
GATCTGGACAGTCTGCAGATCACCGCCGGGGACGACGAAGGCCCGCACGCCGAGCCGGAAATCTCCGGTATCGACGATGC
GCCGATCGTGCGCTTCGTCAACCGTCTTCTGCTGGACGCGGTGCGGAAGGGGGCCTCGGACATCCATATCGAGCCTTACG
AAAAGTTCCTGCGCATCCGCTTCCGGCACGACGGCATCCTGCATGAGGTGGCGAATCCGCCGCCTGCGCTGGGCGTCAAG
ATCTGCGCTCGCATCAAAGTGATGTCGCGCATGGATATCGCGGAACACCGCATTCCCCAGGACGGACGCATCAAGATGAC
GCTGTCGCGCTCCCGTGCCATCGATTTCCGCGTCAATACCTGTCCGACGCTGTTCGGGGAAAAGATCGTGCTGCGTATCC
TCGATCCGAGCTCGGCCCAGATCGGCGTCGAAATGCTGGGATTCGAGCCGGAACAGCGGGACAATTTCCTGCGTGCGCTG
GAAAAGCCCTATGGCATGATCCTGGTTACCGGCCCCACCGGCAGCGGTAAGACCGTTACCCTCTACACCGGTCTGAACCT
CCTCAACCGCGTCGAAGCGAACATCTCCACGGTGGAGGACCCGGTCGAGATCACAGTGCCGGGGATCAACCAGGTCAATG
TCAATTACAAGACCGGCCTCACTTTCGCCGAGGCGCTGCGGGCGTTTCTGCGCCAGGACCCCGACATCATCATGGTGGGC
GAGATCCGCGACCTGGAAACCGCCGAAATCGCCGTCAAGGCGGCCCAGACCGGGCATCTGGTGCTGTCGACCCTGCACAC
CAACGATGCCCCGCAGACCTTGAACCGTCTGATGCAGATGGGGATCCCCGCGTTCAACATCGCCTCCTCCGTGCTGCTGA
TCATGGCGCAGCGGCTGGCCCGCCGGCTTTGTCCGCGGTGCAAGCGGGAGGAGAAAGTGCCGCCCGAGGTCCTGCTCGCC
TCCGGCTTCCGGGAGGAAGACATCGGCAATTTCACGCTGTTCGGGCCGGCGGGCTGCGATCAATGTGTGAAAGGCTACAA
GGGGCGGGTCGGCATTTACCAGGTCATGCCGATTTCGGAAGAAATCAACCGCATCATCCTCGAGGGAGGGAACGTGATGG
CCCTGACCGAGCAGGCGCATGCGGAAGGTATCGCGGATCTGAGGGAGTCTGGCTTGAAGAAGGTGAAGGCGGGTATCACC
AGCCTCGAGGAAATCGACCGCGTGACGAGGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q606C2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.652

100

0.56
  pilB Acinetobacter baylyi ADP1

52.397

100

0.536
  pilB Acinetobacter baumannii D1279779

51.986

100

0.527
  pilB Vibrio cholerae strain A1552

48.845

98.599

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.221

98.424

0.475
  pilF Neisseria gonorrhoeae MS11

47.518

98.774

0.469
  pilB Vibrio campbellii strain DS40M4

47.163

98.774

0.466
  pilF Thermus thermophilus HB27

37.786

99.65

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

40.232

90.543

0.364