Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HQ966_RS02055 Genome accession   NZ_CP078139
Coordinates   445745..446809 (+) Length   354 a.a.
NCBI ID   WP_002551652.1    Uniprot ID   A0A7Z6Y2Y4
Organism   Pseudomonas savastanoi pv. savastanoi strain PVFi1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440745..451809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ966_RS02045 (HQ966_02030) - 441553..442821 (+) 1269 WP_002551650.1 malic enzyme-like NAD(P)-binding protein -
  HQ966_RS02050 (HQ966_02035) - 443094..445535 (-) 2442 WP_369812045.1 penicillin-binding protein 1A -
  HQ966_RS02055 (HQ966_02040) pilM 445745..446809 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  HQ966_RS02060 (HQ966_02045) - 446809..447408 (+) 600 WP_002551653.1 PilN domain-containing protein -
  HQ966_RS02065 (HQ966_02050) pilO 447405..448028 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  HQ966_RS02070 (HQ966_02055) pilP 448025..448552 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  HQ966_RS02075 (HQ966_02060) pilQ 448600..450705 (+) 2106 WP_002551656.1 type IV pilus secretin PilQ Machinery gene
  HQ966_RS02080 (HQ966_02065) aroK 450710..451228 (+) 519 WP_002551657.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38078.57 Da        Isoelectric Point: 4.7008

>NTDB_id=587257 HQ966_RS02055 WP_002551652.1 445745..446809(+) (pilM) [Pseudomonas savastanoi pv. savastanoi strain PVFi1]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=587257 HQ966_RS02055 WP_002551652.1 445745..446809(+) (pilM) [Pseudomonas savastanoi pv. savastanoi strain PVFi1]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGATCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAATGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCTCGTTCTTTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z6Y2Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469