Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   ECTOK1_RS10110 Genome accession   NZ_AP018395
Coordinates   2015993..2017003 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain TOK-001     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2010993..2022003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECTOK1_RS10090 (ECTOK1_C19280) mepM 2011572..2012894 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  ECTOK1_RS10095 (ECTOK1_C19290) znuA 2012910..2013842 (-) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  ECTOK1_RS10100 (ECTOK1_C19300) znuC 2013921..2014676 (+) 756 WP_000202990.1 zinc ABC transporter ATP-binding protein ZnuC -
  ECTOK1_RS10105 (ECTOK1_C19310) znuB 2014673..2015458 (+) 786 WP_000571480.1 zinc ABC transporter permease subunit ZnuB -
  ECTOK1_RS10110 (ECTOK1_C19320) ruvB 2015993..2017003 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  ECTOK1_RS10115 (ECTOK1_C19330) ruvA 2017012..2017623 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  ECTOK1_RS10120 yobI 2017762..2017827 (-) 66 WP_072094247.1 stress response small protein YobI -
  ECTOK1_RS10125 (ECTOK1_C19340) yebB 2017898..2018500 (+) 603 WP_001024907.1 YebB family permuted papain-like enzyme -
  ECTOK1_RS10130 (ECTOK1_C19350) ruvC 2018502..2019023 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  ECTOK1_RS10135 (ECTOK1_C19360) yebC 2019058..2019798 (-) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  ECTOK1_RS10140 (ECTOK1_C19370) nudB 2019827..2020279 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=58625 ECTOK1_RS10110 WP_000568519.1 2015993..2017003(-) (ruvB) [Escherichia coli strain TOK-001]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=58625 ECTOK1_RS10110 WP_000568519.1 2015993..2017003(-) (ruvB) [Escherichia coli strain TOK-001]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGGATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGGGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment