Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   M0D10_RS28975 Genome accession   NZ_CP096207
Coordinates   6176654..6177277 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa TBCF10839     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6171654..6182277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D10_RS28960 (M0D10_28900) - 6172344..6174812 (-) 2469 WP_003114576.1 penicillin-binding protein 1A -
  M0D10_RS28965 (M0D10_28905) pilM 6174997..6176061 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  M0D10_RS28970 (M0D10_28910) pilN 6176061..6176657 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  M0D10_RS28975 (M0D10_28915) pilO 6176654..6177277 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  M0D10_RS28980 (M0D10_28920) comL/pilP 6177274..6177798 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  M0D10_RS28985 (M0D10_28925) - 6177852..6179715 (+) 1864 Protein_5739 type IV pilus secretin PilQ -
  M0D10_RS28990 (M0D10_28930) aroK 6179727..6180245 (+) 519 WP_003095833.1 shikimate kinase AroK -
  M0D10_RS28995 (M0D10_28935) aroB 6180295..6181401 (+) 1107 WP_003162482.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=586187 M0D10_RS28975 WP_003103268.1 6176654..6177277(+) (pilO) [Pseudomonas aeruginosa TBCF10839]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=586187 M0D10_RS28975 WP_003103268.1 6176654..6177277(+) (pilO) [Pseudomonas aeruginosa TBCF10839]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401