Detailed information NTG0586151

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KUU69_RS02045
• Genome accession: NZ_CP078007
• Coordinates: 445246..446394 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain FAHZU40
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 440246..451394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KUU69_RS02020 (KUU69_02020)	-	441397..441693 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
KUU69_RS02025 (KUU69_02025)	-	441690..442283 (-)	594	WP_003084544.1	YggT family protein	-
KUU69_RS02030 (KUU69_02030)	proC	442294..443115 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
KUU69_RS02035 (KUU69_02035)	-	443127..443819 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KUU69_RS02040 (KUU69_02040)	pilT	444034..445068 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KUU69_RS02045 (KUU69_02045)	pilU	445246..446394 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
KUU69_RS02050 (KUU69_02050)	czcD	446402..447301 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KUU69_RS02055 (KUU69_02055)	-	447456..447860 (+)	405	WP_003084560.1	NINE protein	-
KUU69_RS02060 (KUU69_02060)	-	448103..449476 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
KUU69_RS02065 (KUU69_02065)	-	449473..450657 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=586151 KUU69_RS02045 WP_003110016.1 445246..446394(+) (pilU) [Pseudomonas aeruginosa strain FAHZU40]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=586151 KUU69_RS02045 WP_003110016.1 445246..446394(+) (pilU) [Pseudomonas aeruginosa strain FAHZU40]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382