Detailed information NTG0586016

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KUU71_RS02190
• Genome accession: NZ_CP077999
• Coordinates: 469595..470743 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain SRRSH1120
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 464595..475743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KUU71_RS02165 (KUU71_02165)	-	465746..466042 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KUU71_RS02170 (KUU71_02170)	-	466039..466632 (-)	594	WP_003084544.1	YggT family protein	-
KUU71_RS02175 (KUU71_02175)	proC	466643..467464 (-)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
KUU71_RS02180 (KUU71_02180)	-	467476..468168 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KUU71_RS02185 (KUU71_02185)	pilT	468383..469417 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KUU71_RS02190 (KUU71_02190)	pilU	469595..470743 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KUU71_RS02195 (KUU71_02195)	czcD	470751..471650 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KUU71_RS02200 (KUU71_02200)	-	471805..472209 (+)	405	WP_003084560.1	NINE protein	-
KUU71_RS02205 (KUU71_02205)	-	472452..473825 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
KUU71_RS02210 (KUU71_02210)	-	473822..475006 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=586016 KUU71_RS02190 WP_003100959.1 469595..470743(+) (pilU) [Pseudomonas aeruginosa strain SRRSH1120]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=586016 KUU71_RS02190 WP_003100959.1 469595..470743(+) (pilU) [Pseudomonas aeruginosa strain SRRSH1120]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382