Detailed information NTG0585876

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KUU77_RS02330
• Genome accession: NZ_CP077985
• Coordinates: 479721..480869 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ZPPH2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 474721..485869

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KUU77_RS02305 (KUU77_02305)	-	475872..476168 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KUU77_RS02310 (KUU77_02310)	-	476165..476758 (-)	594	WP_003084544.1	YggT family protein	-
KUU77_RS02315 (KUU77_02315)	proC	476769..477590 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
KUU77_RS02320 (KUU77_02320)	-	477602..478294 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KUU77_RS02325 (KUU77_02325)	pilT	478509..479543 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KUU77_RS02330 (KUU77_02330)	pilU	479721..480869 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KUU77_RS02335 (KUU77_02335)	czcD	480877..481776 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KUU77_RS02340 (KUU77_02340)	-	481930..482334 (+)	405	WP_003084560.1	NINE protein	-
KUU77_RS02345 (KUU77_02345)	-	482577..483950 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
KUU77_RS02350 (KUU77_02350)	-	483947..485131 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=585876 KUU77_RS02330 WP_003100959.1 479721..480869(+) (pilU) [Pseudomonas aeruginosa strain ZPPH2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=585876 KUU77_RS02330 WP_003100959.1 479721..480869(+) (pilU) [Pseudomonas aeruginosa strain ZPPH2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGATCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382