Detailed information NTG0585842

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KUU73_RS02050
• Genome accession: NZ_CP077981
• Coordinates: 438548..439696 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ZPPH14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433548..444696

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KUU73_RS02025 (KUU73_02025)	-	434699..434995 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
KUU73_RS02030 (KUU73_02030)	-	434992..435585 (-)	594	WP_003084544.1	YggT family protein	-
KUU73_RS02035 (KUU73_02035)	proC	435596..436417 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
KUU73_RS02040 (KUU73_02040)	-	436429..437121 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
KUU73_RS02045 (KUU73_02045)	pilT	437336..438370 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
KUU73_RS02050 (KUU73_02050)	pilU	438548..439696 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
KUU73_RS02055 (KUU73_02055)	czcD	439704..440603 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
KUU73_RS02060 (KUU73_02060)	-	440757..441161 (+)	405	WP_024918013.1	NINE protein	-
KUU73_RS02065 (KUU73_02065)	-	441404..442777 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
KUU73_RS02070 (KUU73_02070)	-	442774..443958 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=585842 KUU73_RS02050 WP_003100959.1 438548..439696(+) (pilU) [Pseudomonas aeruginosa strain ZPPH14]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=585842 KUU73_RS02050 WP_003100959.1 438548..439696(+) (pilU) [Pseudomonas aeruginosa strain ZPPH14]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382