Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KUU70_RS28125 Genome accession   NZ_CP077977
Coordinates   6005808..6006872 (-) Length   354 a.a.
NCBI ID   WP_023103573.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZPPH29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6000808..6011872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUU70_RS28100 (KUU70_28095) aroK 6001355..6001873 (-) 519 WP_003095833.1 shikimate kinase AroK -
  KUU70_RS28105 (KUU70_28100) pilQ 6001885..6004017 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  KUU70_RS28110 (KUU70_28105) pilP 6004071..6004595 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  KUU70_RS28115 (KUU70_28110) pilO 6004592..6005215 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  KUU70_RS28120 (KUU70_28115) pilN 6005212..6005808 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  KUU70_RS28125 (KUU70_28120) pilM 6005808..6006872 (-) 1065 WP_023103573.1 type IV pilus assembly protein PilM Machinery gene
  KUU70_RS28130 (KUU70_28125) - 6007057..6009528 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -
  KUU70_RS28135 (KUU70_28130) - 6009633..6010901 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37964.43 Da        Isoelectric Point: 4.5399

>NTDB_id=585835 KUU70_RS28125 WP_023103573.1 6005808..6006872(-) (pilM) [Pseudomonas aeruginosa strain ZPPH29]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPEHVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=585835 KUU70_RS28125 WP_023103573.1 6005808..6006872(-) (pilM) [Pseudomonas aeruginosa strain ZPPH29]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCACGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACTGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAAGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466