Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KUU70_RS25390 Genome accession   NZ_CP077977
Coordinates   5396314..5396850 (-) Length   178 a.a.
NCBI ID   WP_025921208.1    Uniprot ID   Q8KQ36
Organism   Pseudomonas aeruginosa strain ZPPH29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5391314..5401850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUU70_RS25370 (KUU70_25365) - 5391889..5394144 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  KUU70_RS25375 (KUU70_25370) nadC 5394430..5395278 (+) 849 WP_017148538.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KUU70_RS25385 (KUU70_25380) tfpZ 5395512..5396249 (-) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  KUU70_RS25390 (KUU70_25385) pilE 5396314..5396850 (-) 537 WP_025921208.1 pilin Machinery gene
  KUU70_RS25395 (KUU70_25390) pilB 5397077..5398780 (+) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KUU70_RS25400 (KUU70_25395) pilC 5398784..5400001 (+) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  KUU70_RS25405 (KUU70_25400) pilD 5400004..5400876 (+) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  KUU70_RS25410 (KUU70_25405) coaE 5400873..5401484 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  KUU70_RS25415 (KUU70_25410) yacG 5401481..5401681 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18322.83 Da        Isoelectric Point: 4.9984

>NTDB_id=585825 KUU70_RS25390 WP_025921208.1 5396314..5396850(-) (pilE) [Pseudomonas aeruginosa strain ZPPH29]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVADQVGLPTAGNTLILSPYINDGNTRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=585825 KUU70_RS25390 WP_025921208.1 5396314..5396850(-) (pilE) [Pseudomonas aeruginosa strain ZPPH29]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGTGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCTTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGACCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACACTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGTTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3JYZ
  PDB 3JZZ

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40.556

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393