Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   MW397_RS19045 Genome accession   NZ_CP095529
Coordinates   4002909..4004402 (-) Length   497 a.a.
NCBI ID   WP_029305422.1    Uniprot ID   -
Organism   Escherichia coli strain GN06156     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3997909..4009402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW397_RS19015 (MW397_19005) gspK 3998703..3999680 (-) 978 WP_029305419.1 type II secretion system minor pseudopilin GspK -
  MW397_RS19020 (MW397_19010) gspJ 3999677..4000282 (-) 606 WP_029305420.1 type II secretion system minor pseudopilin GspJ -
  MW397_RS19025 (MW397_19015) gspI 4000279..4000650 (-) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  MW397_RS19030 (MW397_19020) gspH 4000647..4001210 (-) 564 WP_029305421.1 type II secretion system minor pseudopilin GspH -
  MW397_RS19035 (MW397_19025) gspG 4001214..4001669 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  MW397_RS19040 (MW397_19030) gspF 4001686..4002909 (-) 1224 WP_024242170.1 type II secretion system inner membrane protein GspF -
  MW397_RS19045 (MW397_19035) pilB/pilB1 4002909..4004402 (-) 1494 WP_029305422.1 type II secretion system ATPase GspE Machinery gene
  MW397_RS19050 (MW397_19040) gspD 4004402..4006462 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  MW397_RS19055 (MW397_19045) gspC 4006492..4007451 (-) 960 WP_029305423.1 type II secretion system protein GspC -
  MW397_RS19060 (MW397_19050) gspS2 4007469..4007879 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  MW397_RS19065 (MW397_19055) pilD 4007945..4008754 (-) 810 WP_029305424.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55190.26 Da        Isoelectric Point: 5.9800

>NTDB_id=583780 MW397_RS19045 WP_029305422.1 4002909..4004402(-) (pilB/pilB1) [Escherichia coli strain GN06156]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLNHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=583780 MW397_RS19045 WP_029305422.1 4002909..4004402(-) (pilB/pilB1) [Escherichia coli strain GN06156]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCTCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAACCATGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGTATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGTCTTATGGGGATCTCCGATACGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

40.111

100

0.437
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilF Thermus thermophilus HB27

44.75

80.483

0.36