Detailed information NTG0583670

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KU228_RS14075
• Genome accession: NZ_CP077846
• Coordinates: 2920066..2921184 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain DETAB-P24
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2915066..2926184

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KU228_RS14065 (KU228_14020)	-	2918182..2918874 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
KU228_RS14070 (KU228_14025)	pilT	2919001..2920038 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KU228_RS14075 (KU228_14030)	pilU	2920066..2921184 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KU228_RS14080 (KU228_14035)	fur	2921254..2921691 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KU228_RS14085 (KU228_14040)	bamE	2921803..2922201 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KU228_RS14090 (KU228_14045)	-	2922256..2922579 (-)	324	WP_000442587.1	RnfH family protein	-
KU228_RS14095 (KU228_14050)	-	2922579..2923646 (-)	1068	WP_002019572.1	hypothetical protein	-
KU228_RS14100 (KU228_14055)	-	2923799..2924254 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KU228_RS14105 (KU228_14060)	-	2924507..2925355 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=583670 KU228_RS14075 WP_000347039.1 2920066..2921184(+) (pilU) [Acinetobacter baumannii strain DETAB-P24]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=583670 KU228_RS14075 WP_000347039.1 2920066..2921184(+) (pilU) [Acinetobacter baumannii strain DETAB-P24]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395