Detailed information NTG0583478

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KU217_RS14485
• Genome accession: NZ_CP077835
• Coordinates: 3074975..3076093 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain DETAB-P65
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3069975..3081093

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KU217_RS14475 (KU217_14420)	-	3073091..3073783 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
KU217_RS14480 (KU217_14425)	pilT	3073910..3074947 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KU217_RS14485 (KU217_14430)	pilU	3074975..3076093 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KU217_RS14490 (KU217_14435)	fur	3076163..3076600 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KU217_RS14495 (KU217_14440)	bamE	3076712..3077110 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KU217_RS14500 (KU217_14445)	-	3077165..3077488 (-)	324	WP_000442585.1	RnfH family protein	-
KU217_RS14505 (KU217_14450)	-	3077488..3078555 (-)	1068	WP_004748097.1	hypothetical protein	-
KU217_RS14510 (KU217_14455)	-	3078708..3079163 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KU217_RS14515 (KU217_14460)	-	3079416..3080264 (-)	849	WP_071210971.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=583478 KU217_RS14485 WP_000347034.1 3074975..3076093(+) (pilU) [Acinetobacter baumannii strain DETAB-P65]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=583478 KU217_RS14485 WP_000347034.1 3074975..3076093(+) (pilU) [Acinetobacter baumannii strain DETAB-P65]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39