Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KUE09_RS03580 Genome accession   NZ_CP077801
Coordinates   723811..724929 (-) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain 40288     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 709660..750687 723811..724929 within 0


Gene organization within MGE regions


Location: 709660..750687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUE09_RS03510 (KUE09_03510) - 710493..711539 (-) 1047 WP_001181640.1 hypothetical protein -
  KUE09_RS03515 (KUE09_03515) - 711555..712481 (-) 927 WP_000100974.1 acyltransferase -
  KUE09_RS03520 (KUE09_03520) - 712726..713379 (+) 654 WP_001202415.1 OmpA family protein -
  KUE09_RS03525 (KUE09_03525) rep 713573..715612 (+) 2040 WP_000093035.1 DNA helicase Rep -
  KUE09_RS03530 (KUE09_03530) dut 715636..716088 (+) 453 WP_000868152.1 dUTP diphosphatase -
  KUE09_RS03535 (KUE09_03535) - 716288..717706 (+) 1419 WP_001102836.1 phosphomannomutase/phosphoglucomutase -
  KUE09_RS03540 (KUE09_03540) argB 717721..718629 (+) 909 WP_001135419.1 acetylglutamate kinase -
  KUE09_RS03545 (KUE09_03545) - 718750..719532 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  KUE09_RS03550 (KUE09_03550) - 719641..720489 (+) 849 WP_000336554.1 class II glutamine amidotransferase -
  KUE09_RS03555 (KUE09_03555) - 720741..721196 (+) 456 WP_000782976.1 bacteriohemerythrin -
  KUE09_RS03560 (KUE09_03560) - 721349..722416 (+) 1068 WP_004736014.1 hypothetical protein -
  KUE09_RS03565 (KUE09_03565) - 722416..722739 (+) 324 WP_000442582.1 RnfH family protein -
  KUE09_RS03570 (KUE09_03570) - 722794..723192 (-) 399 WP_001170993.1 outer membrane protein assembly factor BamE -
  KUE09_RS03575 (KUE09_03575) fur 723304..723741 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  KUE09_RS03580 (KUE09_03580) pilU 723811..724929 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KUE09_RS03585 (KUE09_03585) pilT 724957..725994 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  KUE09_RS03590 (KUE09_03590) - 726121..726813 (+) 693 WP_001108513.1 YggS family pyridoxal phosphate-dependent enzyme -
  KUE09_RS03595 (KUE09_03595) - 727014..730610 (-) 3597 WP_000698789.1 SbcC/MukB-like Walker B domain-containing protein -
  KUE09_RS03600 (KUE09_03600) - 730620..731888 (-) 1269 WP_002011272.1 exonuclease SbcCD subunit D -
  KUE09_RS03605 (KUE09_03605) - 732052..732525 (+) 474 WP_000974355.1 OsmC family protein -
  KUE09_RS03610 (KUE09_03610) - 732593..732970 (-) 378 WP_001059437.1 VOC family protein -
  KUE09_RS03615 (KUE09_03615) - 733350..733802 (+) 453 WP_000164152.1 ABZJ_00895 family protein -
  KUE09_RS03620 (KUE09_03620) - 733891..734796 (+) 906 WP_001039352.1 TIGR01777 family oxidoreductase -
  KUE09_RS03625 (KUE09_03625) - 734788..735903 (-) 1116 WP_000546685.1 NAD(P)/FAD-dependent oxidoreductase -
  KUE09_RS03630 (KUE09_03630) hemB 735929..736942 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  KUE09_RS03635 (KUE09_03635) - 737085..737582 (+) 498 WP_002156418.1 thioesterase family protein -
  KUE09_RS03640 (KUE09_03640) - 737852..739003 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  KUE09_RS03645 (KUE09_03645) - 739010..740533 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  KUE09_RS03650 (KUE09_03650) ggt 740838..742823 (+) 1986 WP_001042938.1 gamma-glutamyltransferase -
  KUE09_RS03660 (KUE09_03660) - 743519..744676 (+) 1158 WP_000947475.1 tyrosine-type recombinase/integrase -
  KUE09_RS03665 (KUE09_03665) - 744673..745557 (+) 885 WP_114226310.1 endonuclease/exonuclease/phosphatase family protein -
  KUE09_RS03670 (KUE09_03670) - 745548..746003 (+) 456 WP_000549863.1 hypothetical protein -
  KUE09_RS03675 (KUE09_03675) - 746118..747110 (+) 993 WP_000527288.1 FRG domain-containing protein -
  KUE09_RS03680 (KUE09_03680) - 747117..747287 (-) 171 WP_000119264.1 hypothetical protein -
  KUE09_RS03685 (KUE09_03685) - 747288..747503 (-) 216 WP_039200279.1 hypothetical protein -
  KUE09_RS03690 (KUE09_03690) - 747500..747709 (-) 210 WP_114226311.1 hypothetical protein -
  KUE09_RS03695 (KUE09_03695) - 747706..748155 (-) 450 WP_114226312.1 DUF551 domain-containing protein -
  KUE09_RS03700 (KUE09_03700) - 748159..748404 (-) 246 WP_000654847.1 hypothetical protein -
  KUE09_RS03705 (KUE09_03705) - 748406..749368 (-) 963 WP_114229871.1 hypothetical protein -
  KUE09_RS03710 (KUE09_03710) - 749383..750150 (-) 768 WP_002010120.1 hypothetical protein -
  KUE09_RS03715 (KUE09_03715) - 750162..750485 (-) 324 WP_000181042.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=582962 KUE09_RS03580 WP_000347039.1 723811..724929(-) (pilU) [Acinetobacter baumannii strain 40288]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=582962 KUE09_RS03580 WP_000347039.1 723811..724929(-) (pilU) [Acinetobacter baumannii strain 40288]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395