Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   MUK77_RS03855 Genome accession   NZ_CP095086
Coordinates   794759..796252 (+) Length   497 a.a.
NCBI ID   WP_016238555.1    Uniprot ID   -
Organism   Escherichia coli isolate EC5654     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 789759..801252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUK77_RS03835 (MUK77_03835) pilD 790407..791216 (+) 810 WP_016238557.1 prepilin peptidase PppA Machinery gene
  MUK77_RS03840 (MUK77_03840) gspS2 791282..791692 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  MUK77_RS03845 (MUK77_03845) gspC 791710..792669 (+) 960 WP_024187628.1 type II secretion system protein GspC -
  MUK77_RS03850 (MUK77_03850) gspD 792699..794759 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  MUK77_RS03855 (MUK77_03855) pilB/pilB1 794759..796252 (+) 1494 WP_016238555.1 type II secretion system ATPase GspE Machinery gene
  MUK77_RS03860 (MUK77_03860) gspF 796252..797475 (+) 1224 WP_016238554.1 type II secretion system inner membrane protein GspF -
  MUK77_RS03865 (MUK77_03865) gspG 797492..797947 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  MUK77_RS03870 (MUK77_03870) gspH 797951..798514 (+) 564 WP_016238553.1 type II secretion system minor pseudopilin GspH -
  MUK77_RS03875 (MUK77_03875) gspI 798511..798882 (+) 372 WP_000820112.1 type II secretion system minor pseudopilin GspI -
  MUK77_RS03880 (MUK77_03880) gspJ 798879..799484 (+) 606 WP_016238552.1 type II secretion system minor pseudopilin GspJ -
  MUK77_RS03885 (MUK77_03885) gspK 799481..800458 (+) 978 WP_016238551.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55230.35 Da        Isoelectric Point: 6.0084

>NTDB_id=581992 MUK77_RS03855 WP_016238555.1 794759..796252(+) (pilB/pilB1) [Escherichia coli isolate EC5654]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDDAEFEQRLNAVWQRDSS
ETRQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELLVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=581992 MUK77_RS03855 WP_016238555.1 794759..796252(+) (pilB/pilB1) [Escherichia coli isolate EC5654]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGACGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGACTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
ACAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACAGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACTGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGCTTTCGCGGACGTACTGCCATCCATGAGCTGCTCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.852

86.72

0.38
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45.25

80.483

0.364