Detailed information NTG0058137

Bioinformatically predicted

Overview

• Name: comFC/cflB
• Type: Machinery gene
• Locus tag: SPN994038_RS10720
• Genome accession: NC_021026
• Coordinates: 1993081..1993743 (-)
• Length: 220 a.a.
• NCBI ID: WP_000649971.1
• Uniprot ID: A0A0B7MFB3
• Organism: Streptococcus pneumoniae SPN994038
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1988081..1998743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPN994038_RS10695 (SPN994038_19360)	-	1988231..1988497 (-)	267	WP_001278167.1	Veg family protein	-
SPN994038_RS10700 (SPN994038_19370)	dnaB	1988499..1989851 (-)	1353	WP_000852486.1	replicative DNA helicase	-
SPN994038_RS10705 (SPN994038_19380)	rplI	1989895..1990347 (-)	453	WP_000864220.1	50S ribosomal protein L9	-
SPN994038_RS10710 (SPN994038_19390)	-	1990344..1992317 (-)	1974	WP_000715124.1	DHH family phosphoesterase	-
SPN994038_RS10715 (SPN994038_19400)	hpf	1992453..1993001 (-)	549	WP_000599107.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
SPN994038_RS10720 (SPN994038_19410)	comFC/cflB	1993081..1993743 (-)	663	WP_000649971.1	ComF family protein	Machinery gene
SPN994038_RS10725 (SPN994038_19420)	comFA/cflA	1993740..1995038 (-)	1299	WP_000867612.1	DEAD/DEAH box helicase	Machinery gene
SPN994038_RS10730 (SPN994038_19430)	-	1995094..1995729 (+)	636	WP_000395739.1	YigZ family protein	-
SPN994038_RS10735 (SPN994038_19440)	cysK	1996014..1996934 (+)	921	WP_000029867.1	cysteine synthase A	-
SPN994038_RS10740 (SPN994038_19450)	-	1997173..1997547 (-)	375	Protein_2027	IS66 family transposase	-
SPN994038_RS10745 (SPN994038_19460)	-	1997520..1997807 (+)	288	Protein_2028	transposase	-
SPN994038_RS10750 (SPN994038_19470)	tnpB	1997962..1998285 (-)	324	WP_000973415.1	IS66 family insertion sequence element accessory protein TnpB	-

Sequence

Protein

• Length: 220 a.a.
• Molecular weight: 25241.10 Da
• Isoelectric Point: 7.3688

>NTDB_id=58137 SPN994038_RS10720 WP_000649971.1 1993081..1993743(-) (comFC/cflB) [Streptococcus pneumoniae SPN994038]
MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEENCPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQ
AMKDFFSRYKFDGDFLLRKVFASFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSLVR

Nucleotide

• Length: 663 bp

>NTDB_id=58137 SPN994038_RS10720 WP_000649971.1 1993081..1993743(-) (comFC/cflB) [Streptococcus pneumoniae SPN994038]
ATGAAGTGCTTGTTATGTGGGCAGACTATGAAGACTGTTTTAACTTTTAGTAGTCTCTTACTTCTGAGGAATGATGACTC
TTGTCTTTGTTCAGACTGTGATTCTACTTTTGAAAGAATTGGGGAAGAGAACTGTCCAAATTGTATGAAAACAGAGTTGT
CAACAAAGTGTCAAGATTGTCAACTTTGGTGTAAAGAAGGAGTTGAAGTCAGTCATAGAGCGATTTTTACTTACAATCAA
GCTATGAAGGATTTTTTCAGTCGGTATAAGTTTGATGGAGACTTCCTGTTAAGAAAAGTTTTCGCTTCATTTTTAAGTGA
GGAGTTGAAAAAGTACAAAGAGTATCAATTTGTTGTAATTCCCCTAAGTCCTGATAGATATGCTAACAGAGGATTTAATC
AGGTTGAGGGCTTGGTAGAGGCAGCAGGCTTTGAGTATCTGGATTTATTAGAGAAAAGAGAAGAGAGAGCCAGTTCTTCT
AAAAATCGTTCAGAGCGCTTGGGGACAGAACTTCCTTTCTTTATTAAAAGTGGAGTCACTATTCCTAAAAAAATCCTACT
TATAGATGATATCTATACTACAGGAGCAACTATAAATCGTGTTAAGAAACTGTTGGAAGAAGCTGGTGCTAAGGATGTAA
AAACATTTTCCCTTGTAAGATGA

Domains

Predicted by InterproScan.

• Phosphoribosyl transferase domain (Pribosyltran) (153-219)

3D structure

• AlphaFold DB: A0A0B7MFB3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFC/cflB	Streptococcus pneumoniae TIGR4	100	100	1
comFC/cflB	Streptococcus pneumoniae Rx1	99.545	100	0.995
comFC/cflB	Streptococcus pneumoniae D39	99.545	100	0.995
comFC/cflB	Streptococcus pneumoniae R6	99.545	100	0.995
comFC/cflB	Streptococcus mitis NCTC 12261	91.818	100	0.918
comFC/cflB	Streptococcus mitis SK321	91.364	100	0.914