Detailed information NTG0580914

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6L30_RS06245
• Genome accession: NZ_CP077365
• Coordinates: 1360144..1361262 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain FDAARGOS 1400
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1355144..1366262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6L30_RS06230 (I6L30_06230)	-	1357311..1358153 (-)	843	WP_019456821.1	undecaprenyl-diphosphate phosphatase	-
I6L30_RS06235 (I6L30_06235)	-	1358260..1358952 (-)	693	WP_216985542.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6L30_RS06240 (I6L30_06240)	pilT	1359079..1360116 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
I6L30_RS06245 (I6L30_06245)	pilU	1360144..1361262 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6L30_RS06250 (I6L30_06250)	fur	1361332..1361769 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
I6L30_RS06255 (I6L30_06255)	-	1361881..1362279 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
I6L30_RS06260 (I6L30_06260)	-	1362335..1362658 (-)	324	WP_191108629.1	RnfH family protein	-
I6L30_RS06265 (I6L30_06265)	-	1362658..1363725 (-)	1068	WP_216985543.1	hypothetical protein	-
I6L30_RS06270 (I6L30_06270)	-	1363878..1364333 (-)	456	WP_004703295.1	bacteriohemerythrin	-
I6L30_RS06275 (I6L30_06275)	-	1364584..1365432 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=580914 I6L30_RS06245 WP_004703285.1 1360144..1361262(+) (pilU) [Acinetobacter seifertii strain FDAARGOS 1400]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=580914 I6L30_RS06245 WP_004703285.1 1360144..1361262(+) (pilU) [Acinetobacter seifertii strain FDAARGOS 1400]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGATACTCGTGAATGTAACTTTGCCATCATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAAGTCGGTATTGATACTGACTCGTTTGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAG
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401