Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   I6L86_RS04340 Genome accession   NZ_CP077259
Coordinates   944089..945105 (+) Length   338 a.a.
NCBI ID   WP_004238896.1    Uniprot ID   -
Organism   Streptococcus mitis strain FDAARGOS 1456     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 939089..950105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L86_RS04320 (I6L86_04320) nagA 940237..941388 (+) 1152 WP_001134449.1 N-acetylglucosamine-6-phosphate deacetylase -
  I6L86_RS04325 (I6L86_04325) - 941551..942609 (+) 1059 WP_000649460.1 zinc-dependent alcohol dehydrogenase family protein -
  I6L86_RS04330 (I6L86_04330) - 942759..943124 (+) 366 WP_000286405.1 DUF1033 family protein -
  I6L86_RS04335 (I6L86_04335) comGA/cglA/cilD 943200..944141 (+) 942 WP_000249545.1 competence type IV pilus ATPase ComGA Machinery gene
  I6L86_RS04340 (I6L86_04340) comGB/cglB 944089..945105 (+) 1017 WP_004238896.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6L86_RS04345 (I6L86_04345) comGC/cglC 945107..945430 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  I6L86_RS04350 (I6L86_04350) comGD/cglD 945393..945827 (+) 435 WP_029689647.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6L86_RS04355 (I6L86_04355) comGE/cglE 945790..946092 (+) 303 WP_045597458.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6L86_RS04360 (I6L86_04360) comGF/cglF 946055..946516 (+) 462 WP_000250509.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6L86_RS04365 (I6L86_04365) comGG/cglG 946494..946907 (+) 414 WP_000265657.1 competence type IV pilus minor pilin ComGG Machinery gene
  I6L86_RS04370 (I6L86_04370) - 946940..947527 (+) 588 WP_000679792.1 class I SAM-dependent methyltransferase -
  I6L86_RS04375 (I6L86_04375) comYH 947588..948541 (+) 954 WP_000345104.1 class I SAM-dependent methyltransferase Machinery gene
  I6L86_RS04380 (I6L86_04380) - 948591..949781 (+) 1191 WP_000167778.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38362.31 Da        Isoelectric Point: 9.4588

>NTDB_id=580369 I6L86_RS04340 WP_004238896.1 944089..945105(+) (comGB/cglB) [Streptococcus mitis strain FDAARGOS 1456]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRAGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSVGVLLALTFYKRGSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDMAHALQNGQEFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=580369 I6L86_RS04340 WP_004238896.1 944089..945105(+) (comGB/cglB) [Streptococcus mitis strain FDAARGOS 1456]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
TAATCTCTTTTCCAGCGGTTTTCATCTGGTAGAGACTATCTCCTTTCTAGATAGGAGTGCCTTGTTGGACAAGCAGTGCG
TAACCCAGATGCGTGCGGGCTTATCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCAGAGGTTCATGGGAATCTACACCTGAGTTTGGGAAAGATAGAAGAATATCTGGATAATTT
GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGGTTTCTTCTCTTAATTATGCTGG
GGTTGCGTAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATTATTGGCAATCTGCCACAAATTTTT
CTAGGAATGGTAGGCTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAGGTTCGAAGATGCGGGTCTT
TTCTATCCTGGCACGCATTCCTTTTCTTGGAATTTTTGTGCAGACCTATCTGACAGCCTATTATGCGCGTGAATGGGGCA
ATATGATTTCTCAAGGGATGGAGTTGACGCAGATTTTTCAAATCATGCAGGAACAAGGTTCCCAGCTATTTAAAGAAATT
GGTCAAGATATGGCTCATGCCCTACAAAATGGTCAGGAATTTTCTCAAACCATAGGAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGTTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATCTGGTGCAGCCACTGGTCTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis NCTC 12261

100

100

1
  comGB/cglB Streptococcus mitis SK321

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae Rx1

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae D39

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae R6

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae TIGR4

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

72.321

99.408

0.719
  comYB Streptococcus mutans UA140

59.248

94.379

0.559
  comYB Streptococcus mutans UA159

59.248

94.379

0.559
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5