Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HU725_RS21435 Genome accession   NZ_CP077094
Coordinates   4744434..4745699 (-) Length   421 a.a.
NCBI ID   WP_186476269.1    Uniprot ID   -
Organism   Pseudomonas promysalinigenes strain RW10S1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4739434..4750699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU725_RS21420 (HU725_021420) - 4741090..4742703 (-) 1614 WP_186476271.1 AAA family ATPase -
  HU725_RS21425 (HU725_021425) aroB 4742719..4743816 (-) 1098 WP_186476270.1 3-dehydroquinate synthase -
  HU725_RS21430 (HU725_021430) aroK 4743911..4744429 (-) 519 WP_060479889.1 shikimate kinase AroK -
  HU725_RS21435 (HU725_021435) pilQ 4744434..4745699 (-) 1266 WP_186476269.1 type IV pilus secretin PilQ Machinery gene
  HU725_RS21440 (HU725_021440) - 4745626..4746642 (-) 1017 WP_186476268.1 pilus assembly protein PilP -
  HU725_RS21445 (HU725_021445) - 4746639..4747175 (-) 537 WP_186476267.1 PilN domain-containing protein -
  HU725_RS21450 (HU725_021450) pilM 4747175..4748038 (-) 864 WP_186476266.1 type IV pilus biogenesis protein PilM -
  HU725_RS21455 (HU725_021455) - 4748216..4750669 (+) 2454 WP_060479884.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 45722.49 Da        Isoelectric Point: 5.0981

>NTDB_id=579174 HU725_RS21435 WP_186476269.1 4744434..4745699(-) (pilQ) [Pseudomonas promysalinigenes strain RW10S1]
MRLSKWLVVGLMASQACLAAPFRGEAITLNFQDVEVRSVLQVLADYAGINLVASDTVQGNITLRLHDVPWDQALDLVLHS
KGLARREQGNVLLVAPAAELAEQSRGARIKQELDAQMEPLRRELLPIHHAKASDLAELLLATLADDGILTGRGSLSVDTR
TNTLVAHQPAERLAELRQLVAQLDVPVQQVEIEARIVEANVDYEKGLGVRWGRQLYGDAPLLGKDLFVDLGMDRAAAGIG
VGLLRGGVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDGN
VIMTVKVTKDEPDFVNALNDVPPIRKNEVNAKVRVANGETIVIGGVYSTAQNNVVDKVPFFGDLPYVGRLFRRDALQEKK
SELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=579174 HU725_RS21435 WP_186476269.1 4744434..4745699(-) (pilQ) [Pseudomonas promysalinigenes strain RW10S1]
ATGAGGCTGTCGAAATGGCTAGTGGTGGGCTTGATGGCCAGTCAGGCGTGTCTGGCAGCGCCCTTTCGGGGTGAGGCCAT
AACCCTGAACTTCCAGGATGTGGAGGTGCGCTCAGTGCTTCAGGTGCTGGCTGACTATGCCGGGATCAACTTGGTGGCCA
GTGACACCGTGCAAGGCAATATCACCTTGCGGCTACATGATGTGCCCTGGGATCAAGCGCTGGATTTGGTGTTGCACAGC
AAGGGTCTGGCGCGACGTGAGCAGGGCAATGTGTTGTTGGTCGCGCCCGCCGCAGAGTTGGCCGAGCAGTCTCGCGGTGC
CCGTATCAAACAGGAGCTGGACGCACAAATGGAGCCTTTGCGGCGTGAGCTGCTACCGATTCATCACGCCAAGGCCTCGG
ACCTTGCCGAGTTGCTCCTGGCGACGCTCGCCGATGACGGTATTTTGACCGGCCGTGGCAGTCTGAGTGTCGATACGCGC
ACCAACACCCTGGTAGCGCACCAGCCGGCTGAGCGCCTGGCCGAGTTGCGCCAGTTGGTCGCACAGCTTGATGTACCGGT
CCAGCAGGTGGAAATCGAGGCGCGTATCGTGGAGGCCAATGTCGATTACGAAAAAGGCCTGGGGGTGCGCTGGGGGCGGC
AGTTATACGGGGATGCACCACTGTTGGGCAAGGACCTGTTCGTCGACCTGGGCATGGACCGGGCCGCAGCTGGCATTGGC
GTAGGGTTGTTGCGCGGCGGTGTGCTGCTGGACCTGGAGCTCAGCGCGATGGAGAAAAGCGGCAACGGCGAAATCATTTC
CCAGCCCAAGGTGGTTACGGCTGACAAGGAAACCGCCAGGATTCTCAAAGGCACCGAAGTGCCCTACCAGGAAACCAGCA
AAAGCGGGGCGACCTCGGTAGCCTTTCGCGAAGCCTCGCTGTCACTGGAAGTTACTCCACAGATCACGCCGGACGGCAAT
GTCATCATGACGGTCAAAGTGACCAAGGATGAGCCCGATTTCGTCAATGCTCTCAACGACGTACCACCGATTCGCAAGAA
CGAAGTCAATGCCAAGGTTCGGGTAGCGAATGGGGAGACCATCGTCATCGGTGGCGTTTACTCGACCGCGCAAAACAATG
TGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTATTTCGACGCGATGCATTACAAGAGAAAAAA
TCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(124-189)

Bacterial type II and III secretion system protein (Secretin)

(256-412)

Secretin and TonB N terminus short domain (STN)

(50-96)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.184

100

0.62
  comQ Acinetobacter baylyi ADP1

42.572

100

0.456
  pilQ Acinetobacter baumannii D1279779

41.266

100

0.449
  pilQ Acinetobacter baumannii strain A118

41.266

100

0.449
  pilQ Legionella pneumophila strain ERS1305867

44.019

99.287

0.437
  pilQ Legionella pneumophila strain Lp02

44.019

99.287

0.437
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.269

98.812

0.428
  pilQ Vibrio cholerae strain A1552

43.269

98.812

0.428
  pilQ Vibrio campbellii strain DS40M4

41.371

100

0.416
  pilQ Neisseria meningitidis MC58

37.709

99.525

0.375
  pilQ Neisseria meningitidis M1080

37.709

99.525

0.375
  pilQ Neisseria gonorrhoeae strain FA1090

37.47

99.525

0.373