Detailed information NTG0578288

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KQ255_RS06270
• Genome accession: NZ_CP076821
• Coordinates: 1310675..1311793 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain UC22850
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1305675..1316793

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KQ255_RS06240 (KQ255_06215)	-	1306504..1307352 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
KQ255_RS06245 (KQ255_06220)	-	1307605..1308060 (+)	456	WP_000782976.1	bacteriohemerythrin	-
KQ255_RS06250 (KQ255_06225)	-	1308213..1309280 (+)	1068	WP_000092403.1	hypothetical protein	-
KQ255_RS06255 (KQ255_06230)	-	1309280..1309603 (+)	324	WP_000442585.1	RnfH family protein	-
KQ255_RS06260 (KQ255_06235)	bamE	1309658..1310056 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KQ255_RS06265 (KQ255_06240)	fur	1310168..1310605 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KQ255_RS06270 (KQ255_06245)	pilU	1310675..1311793 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KQ255_RS06275 (KQ255_06250)	pilT	1311821..1312858 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
KQ255_RS06280 (KQ255_06255)	-	1312985..1313677 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=578288 KQ255_RS06270 WP_000347036.1 1310675..1311793(-) (pilU) [Acinetobacter baumannii strain UC22850]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=578288 KQ255_RS06270 WP_000347036.1 1310675..1311793(-) (pilU) [Acinetobacter baumannii strain UC22850]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392