Detailed information NTG0578251

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KQ257_RS14825
• Genome accession: NZ_CP076817
• Coordinates: 3077930..3079048 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347040.1
• Uniprot ID: A0A809JLP7
• Organism: Acinetobacter baumannii strain UC24137
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3072930..3084048

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KQ257_RS14795 (KQ257_14735)	-	3073759..3074607 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
KQ257_RS14800 (KQ257_14740)	-	3074860..3075315 (+)	456	WP_000782976.1	bacteriohemerythrin	-
KQ257_RS14805 (KQ257_14745)	-	3075468..3076535 (+)	1068	WP_046693192.1	membrane protein	-
KQ257_RS14810 (KQ257_14750)	-	3076535..3076858 (+)	324	WP_000442590.1	RnfH family protein	-
KQ257_RS14815 (KQ257_14755)	bamE	3076913..3077311 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KQ257_RS14820 (KQ257_14760)	fur	3077423..3077860 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KQ257_RS14825 (KQ257_14765)	pilU	3077930..3079048 (-)	1119	WP_000347040.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KQ257_RS14830 (KQ257_14770)	pilT	3079076..3080113 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KQ257_RS14835 (KQ257_14775)	-	3080240..3080932 (+)	693	WP_004748094.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41896.10 Da
• Isoelectric Point: 6.6406

>NTDB_id=578251 KQ257_RS14825 WP_000347040.1 3077930..3079048(-) (pilU) [Acinetobacter baumannii strain UC24137]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=578251 KQ257_RS14825 WP_000347040.1 3077930..3079048(-) (pilU) [Acinetobacter baumannii strain UC24137]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A809JLP7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392