Detailed information NTG0577872

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: MTO79_RS00015
• Genome accession: NZ_CP094474
• Coordinates: 1609..2682 (-)
• Length: 357 a.a.
• NCBI ID: WP_014570824.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain SCB469
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1..7682

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTO79_RS00005 (MTO79_00005)	-	1..1458 (+)	1458	WP_058221720.1	recombinase family protein	-
MTO79_RS00010 (MTO79_00010)	-	1455..1595 (-)	141	WP_023164653.1	hypothetical protein	-
MTO79_RS00015 (MTO79_00015)	comGB	1609..2682 (-)	1074	WP_014570824.1	competence type IV pilus assembly protein ComGB	Machinery gene
MTO79_RS00020 (MTO79_00020)	comGA	2576..3515 (-)	940	Protein_3	competence type IV pilus ATPase ComGA	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40366.59 Da
• Isoelectric Point: 10.4062

>NTDB_id=577872 MTO79_RS00015 WP_014570824.1 1609..2682(-) (comGB) [Lactococcus lactis strain SCB469]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=577872 MTO79_RS00015 WP_014570824.1 1609..2682(-) (comGB) [Lactococcus lactis strain SCB469]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACCTTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	82.698	95.518	0.79
comGB	Streptococcus gordonii str. Challis substr. CH1	53.666	95.518	0.513
comGB	Streptococcus mutans UA140	53.372	95.518	0.51
comGB	Streptococcus mutans UA159	53.079	95.518	0.507
comGB/cglB	Streptococcus pneumoniae TIGR4	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae D39	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae R6	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae Rx1	51.343	93.838	0.482
comGB/cglB	Streptococcus mitis SK321	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis NCTC 12261	50.149	93.838	0.471