Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   LEUCM_RS08730 Genome accession   NZ_AP017929
Coordinates   1742311..1744194 (-) Length   627 a.a.
NCBI ID   WP_025015867.1    Uniprot ID   -
Organism   Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1737311..1749194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEUCM_RS08705 (LACBS_01730) - 1738790..1739152 (-) 363 WP_016264868.1 Asp23/Gls24 family envelope stress response protein -
  LEUCM_RS08710 (LACBS_01731) rpmB 1739455..1739640 (+) 186 WP_004265581.1 50S ribosomal protein L28 -
  LEUCM_RS08715 (LACBS_01732) - 1739989..1740639 (-) 651 WP_016264867.1 thiamine diphosphokinase -
  LEUCM_RS08720 (LACBS_01733) rpe 1740626..1741288 (-) 663 WP_016264866.1 ribulose-phosphate 3-epimerase -
  LEUCM_RS08725 (LACBS_01734) rsgA 1741312..1742211 (-) 900 WP_011374402.1 ribosome small subunit-dependent GTPase A -
  LEUCM_RS08730 (LACBS_01735) pknB 1742311..1744194 (-) 1884 WP_025015867.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  LEUCM_RS08735 (LACBS_01736) - 1744191..1744937 (-) 747 WP_025015868.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  LEUCM_RS08740 (LACBS_01737) rsmB 1744959..1746302 (-) 1344 WP_056936395.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LEUCM_RS08745 (LACBS_01738) fmt 1746292..1747248 (-) 957 WP_025015869.1 methionyl-tRNA formyltransferase -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 68228.85 Da        Isoelectric Point: 5.9848

>NTDB_id=57705 LEUCM_RS08730 WP_025015867.1 1742311..1744194(-) (pknB) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
MMETGYAVAGRYKIIRPIGEGGMANVYLAQDLILDRQVAVKVLRLDLRNDPDTVRRFTREALATTELNHPNIVSIYDVGE
ENSMQYIIMEYIKGTDLKKYIVEHFPIPYQRVIDIMTQILSAVQNAHAHNIIHRDLKPQNILVDEDGNVKISDFGIAIAL
SETAMTQTNTLLGSVHYLSPEQARGSMATKRSDIYSLGIVLYELLTGMVPFEGESAVSIAIKHFQDEVPPVRNYDPRIPQ
ALENVVLKATAKDPDERYSGVDAMMADLATSLSASRAHEPKFVPSKADDLSETKVIPALDPEGITQTNNDEEQVKEAEPT
QKKVKKPAKSKRKWWLLGGGVVLLLLLATVIAFAVQGSEVTVPDLTDMTQQNAESALTDKKLKVGTVQKTTSQKYSKGHV
IRTTPKAGLSVKSRSTVNLIVSSGRQKYKIKDYTNQAYSDVRDALKDKGLKVKRKYQSSSEVSPGLIIDQSIAAGKRVVP
SKTTITLTVSSGKPGFALHNLSNYTKQGIQDYAAQNGLTVVFSEEYSSSISEGMVISQSPASGTMVQEGDSVSVILSKGE
QPTEHHSIVTSSSNSVIESSAPESSQSSESSASESESESESESESESASESISSSASQSSQSSVQSK

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=57705 LEUCM_RS08730 WP_025015867.1 1742311..1744194(-) (pknB) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
ATGATGGAAACAGGGTACGCTGTAGCGGGGCGTTATAAGATTATTCGCCCCATCGGTGAAGGTGGCATGGCTAATGTTTA
TTTGGCGCAGGATCTAATTTTAGATCGGCAAGTGGCAGTTAAAGTTTTACGATTAGATCTCCGAAATGATCCGGATACAG
TCCGACGGTTTACGCGAGAAGCGTTGGCAACGACTGAATTAAATCATCCGAATATTGTTAGTATTTACGATGTTGGTGAA
GAAAACAGTATGCAATACATCATTATGGAATATATTAAGGGCACCGATCTTAAGAAATATATCGTGGAACACTTTCCAAT
TCCTTATCAACGCGTAATTGATATTATGACGCAGATTTTATCTGCGGTTCAAAACGCACATGCGCATAATATTATTCATC
GGGATTTAAAACCACAAAATATTTTGGTTGATGAAGACGGCAACGTTAAGATTAGTGACTTTGGGATTGCGATTGCGCTC
AGCGAAACGGCGATGACTCAGACCAATACGTTGTTGGGGTCTGTTCATTATCTCTCACCGGAACAGGCAAGAGGTTCGAT
GGCGACGAAACGGTCTGATATTTACTCGTTAGGAATTGTGCTTTATGAGTTATTGACGGGGATGGTGCCTTTTGAAGGCG
AGTCCGCTGTTTCAATTGCGATTAAGCATTTCCAAGATGAAGTCCCACCAGTACGCAACTATGATCCACGTATTCCGCAA
GCCTTGGAAAATGTCGTTTTAAAAGCGACTGCCAAGGATCCGGATGAACGCTATAGTGGTGTTGATGCAATGATGGCTGA
CTTAGCAACTTCTTTATCAGCTAGTCGTGCGCACGAACCTAAATTTGTGCCATCAAAAGCGGATGATTTGAGTGAGACTA
AGGTGATTCCCGCACTAGATCCAGAAGGCATTACGCAAACCAACAATGATGAGGAACAAGTTAAAGAAGCGGAACCAACT
CAGAAAAAGGTTAAAAAGCCAGCTAAGTCTAAACGTAAGTGGTGGCTACTAGGTGGCGGTGTTGTTTTATTGCTGTTATT
AGCAACGGTGATTGCTTTTGCAGTTCAGGGGAGCGAAGTAACCGTCCCTGATTTAACGGATATGACACAACAAAATGCGG
AATCTGCTTTGACCGATAAAAAACTGAAGGTCGGCACGGTTCAAAAAACAACGAGTCAGAAATACAGTAAGGGCCACGTC
ATTCGAACAACGCCTAAAGCGGGCCTGAGTGTAAAATCAAGAAGTACGGTTAATTTAATTGTCAGTTCAGGACGCCAAAA
GTATAAGATTAAAGACTATACGAACCAAGCATATAGTGATGTTCGGGATGCTTTGAAGGATAAAGGCTTGAAAGTTAAAC
GGAAATACCAAAGTTCATCTGAAGTGAGTCCTGGGTTGATTATCGATCAAAGTATTGCGGCTGGCAAAAGGGTCGTTCCA
AGTAAAACGACGATTACCTTAACAGTCAGCAGCGGCAAACCTGGTTTTGCATTGCATAATCTAAGCAACTATACAAAGCA
AGGGATTCAAGACTATGCTGCACAAAATGGTTTAACGGTCGTATTCTCTGAAGAATATTCATCAAGTATTTCTGAAGGAA
TGGTAATCAGTCAATCGCCTGCAAGTGGTACAATGGTTCAAGAAGGTGATAGCGTAAGCGTTATCCTTTCAAAAGGCGAG
CAACCAACTGAACATCATTCTATTGTGACGTCGAGTTCAAATTCAGTTATCGAGTCGAGTGCGCCTGAATCAAGTCAATC
GAGTGAAAGTAGCGCGAGTGAGTCAGAGAGCGAATCAGAGAGTGAATCGGAAAGCGAATCAGCAAGTGAATCGATCAGTA
GTTCAGCTAGTCAAAGTAGTCAATCGAGCGTTCAATCAAAGTAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-263)

PASTA domain (PASTA)

(429-490)

PASTA domain (PASTA)

(360-422)

PASTA domain (PASTA)

(500-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

41.88

93.301

0.391
  stkP Streptococcus pneumoniae TIGR4

40.304

94.577

0.381
  stkP Streptococcus pneumoniae D39

40.135

94.577

0.38
  stkP Streptococcus pneumoniae R6

40.135

94.577

0.38
  stkP Streptococcus pneumoniae Rx1

39.966

94.577

0.378

Multiple sequence alignment