Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KP808_RS01965 Genome accession   NZ_CP076652
Coordinates   428200..429264 (+) Length   354 a.a.
NCBI ID   WP_002551652.1    Uniprot ID   A0A7Z6Y2Y4
Organism   Pseudomonas savastanoi strain MHT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423200..434264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP808_RS01960 (KP808_01945) - 425549..427990 (-) 2442 WP_407692673.1 penicillin-binding protein 1A -
  KP808_RS01965 (KP808_01950) pilM 428200..429264 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  KP808_RS01970 (KP808_01955) - 429264..429863 (+) 600 WP_004654254.1 PilN domain-containing protein -
  KP808_RS01975 (KP808_01960) pilO 429860..430483 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  KP808_RS01980 (KP808_01965) pilP 430480..431007 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  KP808_RS01985 (KP808_01970) pilQ 431055..433160 (+) 2106 WP_005743117.1 type IV pilus secretin PilQ Machinery gene
  KP808_RS01990 (KP808_01975) aroK 433165..433683 (+) 519 WP_058399805.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38078.57 Da        Isoelectric Point: 4.7008

>NTDB_id=576951 KP808_RS01965 WP_002551652.1 428200..429264(+) (pilM) [Pseudomonas savastanoi strain MHT1]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=576951 KP808_RS01965 WP_002551652.1 428200..429264(+) (pilM) [Pseudomonas savastanoi strain MHT1]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGCCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATTGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAGGTATTGCTGGCTGCCTGCCGCAAAGAGAACGTCGAAGTGCGGGAGGCAGCGCTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAAGCTTACGCCCTTGAACGCTCGTTCGGGCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGAGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGCCGCTATGGCCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCCCGTTCTTTGCAGTTCTTTTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGCATTGGCACCCCTACGATGGTCGCCAACCCG
TTTGCCGATATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCACTGGCCAGTGACGCTCCCGCATTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z6Y2Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469