Detailed information NTG0576592

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KMZ14_RS07525
• Genome accession: NZ_CP076603
• Coordinates: 1505333..1506511 (+)
• Length: 392 a.a.
• NCBI ID: WP_273798993.1
• Uniprot ID: -
• Organism: Acinetobacter schindleri strain MCDA01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1500333..1511511

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KMZ14_RS07510 (KMZ14_07500)	-	1501669..1502781 (+)	1113	WP_273798991.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
KMZ14_RS07515 (KMZ14_07505)	-	1502987..1503517 (+)	531	WP_004809525.1	hypothetical protein	-
KMZ14_RS07520 (KMZ14_07510)	-	1504039..1505088 (+)	1050	WP_273798992.1	3-deoxy-7-phosphoheptulonate synthase	-
KMZ14_RS07525 (KMZ14_07515)	pilU	1505333..1506511 (+)	1179	WP_273798993.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KMZ14_RS07530 (KMZ14_07520)	pilU	1506920..1508125 (+)	1206	WP_273798995.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KMZ14_RS07535 (KMZ14_07525)	-	1508184..1509362 (-)	1179	WP_273798996.1	MFS transporter	-
KMZ14_RS07540 (KMZ14_07530)	gcvH	1509593..1509967 (-)	375	WP_004809515.1	glycine cleavage system protein GcvH	-
KMZ14_RS07545 (KMZ14_07535)	-	1510040..1511068 (-)	1029	WP_004809513.1	AraC family transcriptional regulator	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 44480.12 Da
• Isoelectric Point: 6.5062

>NTDB_id=576592 KMZ14_RS07525 WP_273798993.1 1505333..1506511(+) (pilU) [Acinetobacter schindleri strain MCDA01]
MYTPELLDEARKFMHHMLTKVVEYGGSDLFITADFPPSIKQQGLMKPFGQQKLAADKTRLFAYALMNERQIKEFESVMEC
NFGISVPGVSRFRVNVFQQQHNVGMVIRTITTEIPSFQQLGLPNSLQQVIMEKRGLVLIVGATGVGKSTTLAAMIDHRNT
YSEGHIITMEDPVEYVYQHKKSMITQREIGVDCVSWHNALINTMRQAPDVIVIGEIRDTESMEHAIAFAESGHLCLATLH
SNNADQALDRIINFFPEERRNQLLMDLSANMKAVISQRLIRTEDGQGRRAAVEILLNTPLVSDFIRKGDFYELKTIMARS
RELGMQTFDQALFDLYNQGVISYDEALRNADSSNEMRLQIKLKSSRINPALQEEGSKMTILEQSKEITLSRI

Nucleotide

• Length: 1179 bp

>NTDB_id=576592 KMZ14_RS07525 WP_273798993.1 1505333..1506511(+) (pilU) [Acinetobacter schindleri strain MCDA01]
ATGTATACACCCGAATTGCTCGATGAAGCACGTAAATTTATGCACCATATGCTGACCAAAGTGGTTGAATATGGAGGTTC
CGATCTTTTTATTACCGCAGATTTTCCACCAAGTATCAAACAGCAGGGCTTGATGAAACCTTTTGGTCAGCAAAAACTGG
CAGCGGATAAAACCCGTTTGTTTGCCTATGCTTTAATGAATGAACGACAGATCAAGGAATTTGAATCAGTGATGGAATGC
AATTTCGGTATCAGTGTACCGGGTGTATCACGCTTCCGGGTCAATGTCTTTCAGCAACAGCATAATGTTGGGATGGTAAT
CAGAACCATTACTACGGAGATTCCAAGCTTTCAGCAATTAGGACTACCAAACTCTTTGCAACAAGTGATTATGGAAAAAC
GTGGGCTGGTTCTGATTGTAGGTGCGACAGGTGTTGGTAAATCCACCACACTGGCAGCCATGATTGATCACCGCAATACC
TATTCTGAAGGACATATTATTACCATGGAAGATCCGGTGGAATACGTGTATCAACATAAAAAGTCCATGATTACCCAACG
TGAAATTGGCGTAGATTGTGTTTCCTGGCATAACGCGCTGATCAATACCATGCGTCAGGCACCGGATGTGATCGTGATTG
GTGAAATCCGGGATACCGAAAGTATGGAGCATGCGATTGCCTTTGCTGAATCCGGTCATCTGTGTTTGGCCACCCTGCAT
TCTAATAATGCCGATCAGGCTTTAGACCGCATTATTAATTTCTTCCCGGAAGAACGCCGCAATCAGCTGTTAATGGATTT
ATCCGCCAATATGAAAGCTGTGATTTCGCAACGTTTGATCCGTACCGAGGATGGGCAAGGTCGTCGTGCTGCTGTTGAAA
TTTTATTGAATACGCCGCTGGTGTCTGATTTTATCCGTAAAGGAGATTTTTACGAGCTGAAAACTATTATGGCGCGTTCG
CGTGAACTCGGCATGCAGACCTTTGATCAGGCATTATTTGATTTGTATAACCAGGGAGTTATTTCTTATGACGAAGCCTT
GCGTAATGCAGATTCAAGTAATGAAATGCGTTTGCAGATCAAATTAAAGAGCAGTCGCATCAATCCGGCTCTTCAGGAAG
AAGGAAGTAAAATGACGATACTGGAACAGTCTAAGGAAATTACACTTTCCAGAATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (64-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.08	95.408	0.526
pilU	Acinetobacter baylyi ADP1	55.085	90.306	0.497
pilU	Vibrio cholerae strain A1552	49.859	90.561	0.452
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.773	86.48	0.37