Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CLST_RS05385 Genome accession   NC_020887
Coordinates   1252098..1253006 (+) Length   302 a.a.
NCBI ID   WP_015358829.1    Uniprot ID   L7VJB0
Organism   Thermoclostridium stercorarium subsp. stercorarium DSM 8532     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1247098..1258006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLST_RS05380 (Clst_1095) smc 1248447..1252025 (+) 3579 WP_041746643.1 chromosome segregation protein SMC -
  CLST_RS05385 (Clst_1096) pilA 1252098..1253006 (+) 909 WP_015358829.1 signal recognition particle-docking protein FtsY Machinery gene
  CLST_RS05390 (Clst_1097) - 1253147..1254640 (+) 1494 WP_015358830.1 IMP dehydrogenase -
  CLST_RS05395 (Clst_1098) - 1254840..1255598 (+) 759 WP_015358831.1 ATP-dependent Clp protease proteolytic subunit -
  CLST_RS05400 (Clst_1099) - 1255595..1255861 (+) 267 WP_015358832.1 YlzJ-like family protein -
  CLST_RS05405 (Clst_1102) serA 1256330..1257934 (+) 1605 WP_015358833.1 phosphoglycerate dehydrogenase -

Sequence

Protein


Download         Length: 302 a.a.        Molecular weight: 32792.05 Da        Isoelectric Point: 5.8071

>NTDB_id=57609 CLST_RS05385 WP_015358829.1 1252098..1253006(+) (pilA) [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
MSFFEKLKNGLKKTRESITQRIDQLLVSMGKIDEELFEELEEILITSDVGVETTLKIIDDLKLRVKSEKVTDPRQVKNLL
KEEITSILSSGDTGLHLNTKPSVIVVVGVNGVGKTTSIGKMCHMLKMQGKKVLVAAGDTFRAAAIDQLEIWAKRAGVDII
KHAEGSDPSAVIFDAIQAAKARGYDVVICDTAGRLHTKKNLMEELKKVFRIISRELPDADIETLLVLDATTGQNAVSQAR
TFAEATGVTGLILTKLDGTAKGGIIVSIKSELDIPVKFIGVGEKIDDLQPFDAASFAEALFS

Nucleotide


Download         Length: 909 bp        

>NTDB_id=57609 CLST_RS05385 WP_015358829.1 1252098..1253006(+) (pilA) [Thermoclostridium stercorarium subsp. stercorarium DSM 8532]
ATGTCTTTTTTTGAGAAACTGAAAAACGGTCTGAAAAAAACAAGGGAAAGCATTACACAAAGAATTGATCAGTTGCTTGT
TTCAATGGGCAAAATAGATGAGGAGCTATTTGAAGAGCTGGAGGAAATACTTATTACCTCCGACGTGGGCGTTGAAACAA
CGCTTAAAATAATTGATGATTTGAAACTAAGGGTAAAAAGCGAGAAAGTAACCGATCCCAGGCAGGTAAAAAATCTGTTA
AAGGAAGAAATAACCAGTATTTTAAGTTCGGGTGACACCGGGCTGCACCTTAACACAAAGCCTTCGGTAATTGTGGTGGT
TGGTGTCAATGGCGTGGGCAAAACCACATCAATAGGCAAGATGTGCCATATGCTGAAAATGCAGGGGAAAAAGGTTCTCG
TAGCAGCAGGTGATACTTTCAGGGCGGCGGCGATAGATCAGCTTGAAATATGGGCTAAAAGGGCAGGGGTGGATATAATA
AAGCATGCCGAGGGTTCTGATCCGTCAGCGGTTATTTTTGACGCAATTCAGGCGGCAAAGGCACGGGGATATGATGTGGT
GATCTGTGACACCGCGGGGCGTCTGCATACCAAAAAGAACCTGATGGAAGAGCTCAAGAAGGTTTTCAGGATTATCAGCC
GGGAACTCCCCGATGCGGATATTGAAACACTTCTGGTCCTTGATGCTACGACAGGTCAGAACGCCGTCTCGCAGGCGAGA
ACCTTTGCCGAAGCCACCGGTGTTACGGGACTGATTCTTACAAAGCTTGACGGTACGGCAAAAGGAGGAATTATAGTATC
AATTAAGTCTGAGCTTGACATTCCGGTAAAATTCATAGGTGTCGGGGAAAAAATAGATGATCTGCAGCCTTTTGACGCCG
CAAGTTTTGCCGAAGCTTTGTTTTCATAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(101-301)

SRP54-type protein, helical bundle domain (SRP54_N)

(19-84)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L7VJB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.185

100

0.483

Multiple sequence alignment