Detailed information NTG0057457

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H681_RS13230
• Genome accession: NC_020829
• Coordinates: 2987877..2989091 (-)
• Length: 404 a.a.
• NCBI ID: WP_015477374.1
• Uniprot ID: -
• Organism: Pseudomonas sp. ATCC 13867
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2982877..2994091

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H681_RS13215 (H681_13215)	-	2983953..2985395 (-)	1443	WP_015477371.1	alanine/glycine:cation symporter family protein	-
H681_RS13220 (H681_13220)	-	2985563..2986381 (+)	819	WP_015477372.1	helix-turn-helix transcriptional regulator	-
H681_RS13225 (H681_13225)	-	2986503..2987387 (-)	885	WP_015477373.1	DMT family transporter	-
H681_RS13230 (H681_13230)	pilU	2987877..2989091 (-)	1215	WP_015477374.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H681_RS13235 (H681_13235)	-	2989279..2989557 (+)	279	WP_015477375.1	peptidylprolyl isomerase	-
H681_RS13240 (H681_13240)	-	2989565..2989930 (+)	366	WP_015477376.1	MmcQ/YjbR family DNA-binding protein	-
H681_RS13245 (H681_13245)	-	2989927..2990481 (+)	555	WP_015477377.1	putative glycolipid-binding domain-containing protein	-
H681_RS13250 (H681_13250)	-	2990814..2991986 (+)	1173	WP_015477378.1	MFS transporter	-
H681_RS13255 (H681_13255)	-	2991983..2993632 (-)	1650	WP_041711990.1	phosphoethanolamine--lipid A transferase	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 44375.73 Da
• Isoelectric Point: 6.2535

>NTDB_id=57457 H681_RS13230 WP_015477374.1 2987877..2989091(-) (pilU) [Pseudomonas sp. ATCC 13867]
MDIQSMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSNDPLKAGDVARIGYSLMDEEQRAEFERELEMNLAISLQGVGR
FRINLFKQRNEVSIVARNIKLDIPLFADLKLPDVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFNSIKEIMDKSRALGMQTFDQAL
FDLAVEGAITEDEAVKNADSANNLRLKLKLYKDNPAMTTHSVAASPAPAPVAAPAPVLEASNWGLELALEEIEAEDAEGK
PVGQ

Nucleotide

• Length: 1215 bp

>NTDB_id=57457 H681_RS13230 WP_015477374.1 2987877..2989091(-) (pilU) [Pseudomonas sp. ATCC 13867]
ATGGATATCCAGTCGATGCTGAAGATCCTGGCCACCCAGGATGGTTCCGACCTCTATCTTTCCACCGGTGCGCCGCCTTG
CGCCAAGTTCAACGGCGTACTCAAGCCGCTGTCCAACGATCCGCTCAAGGCCGGCGACGTGGCGCGCATCGGCTACAGCC
TGATGGACGAGGAGCAGCGCGCCGAATTTGAGCGCGAACTGGAGATGAACCTGGCCATTTCGCTGCAGGGCGTGGGTCGT
TTCCGCATCAACCTGTTCAAGCAGCGCAACGAAGTCTCCATCGTGGCGCGCAACATCAAGCTGGACATCCCACTGTTCGC
CGACCTGAAACTGCCGGACGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCATATCATCACCATCGAAGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAGGTGGGCGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACGATGGAGCACG
CCCTGGCCTTCGCCGACACCGGTCACCTGGCGATCTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGAACCCAGGACGGCAAGCGTCGCGCCGCAGTGGAGGTGCTGCTGGGCACGGCGACCATCAGCGATATCATCA
AGCGCGGCGACTTCAATTCGATCAAGGAGATCATGGACAAGTCCCGCGCGCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTCGCCGTGGAAGGGGCGATCACCGAGGACGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGCCTGAA
GCTCAAGCTCTACAAGGACAACCCGGCGATGACGACCCACTCCGTCGCCGCCAGCCCGGCACCGGCGCCCGTCGCGGCGC
CTGCCCCGGTGCTGGAGGCGTCCAATTGGGGGCTGGAGCTGGCGCTGGAAGAAATCGAGGCCGAGGACGCGGAAGGCAAG
CCGGTCGGGCAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (109-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	86.881	0.49
pilU	Acinetobacter baylyi ADP1	53.239	87.871	0.468
pilU	Vibrio cholerae strain A1552	50.571	86.634	0.438
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.8	92.822	0.379