Detailed information NTG0057449

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H681_RS04955
• Genome accession: NC_020829
• Coordinates: 1071346..1072218 (-)
• Length: 290 a.a.
• NCBI ID: WP_015475737.1
• Uniprot ID: M4XC34
• Organism: Pseudomonas sp. ATCC 13867
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1066346..1077218

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H681_RS04915 (H681_04885)	-	1067459..1067629 (-)	171	WP_015475729.1	DUF3094 domain-containing protein	-
H681_RS04920 (H681_04890)	-	1067699..1068328 (-)	630	WP_015475730.1	DUF1780 domain-containing protein	-
H681_RS04925 (H681_04895)	-	1068438..1068854 (+)	417	WP_015475731.1	GNAT family N-acetyltransferase	-
H681_RS04930 (H681_04900)	-	1068857..1069324 (+)	468	WP_041711734.1	hypothetical protein	-
H681_RS04935 (H681_04905)	-	1069321..1070010 (+)	690	WP_015475733.1	energy-coupling factor ABC transporter permease	-
H681_RS04940 (H681_04910)	-	1070117..1070332 (+)	216	WP_015475734.1	hypothetical protein	-
H681_RS04945 (H681_04915)	yacG	1070472..1070675 (-)	204	WP_015475735.1	DNA gyrase inhibitor YacG	-
H681_RS04950 (H681_04920)	coaE	1070672..1071280 (-)	609	WP_015475736.1	dephospho-CoA kinase	-
H681_RS04955 (H681_04925)	pilD	1071346..1072218 (-)	873	WP_015475737.1	A24 family peptidase	Machinery gene
H681_RS04960 (H681_04930)	pilC	1072224..1073441 (-)	1218	WP_015475738.1	type II secretion system F family protein	Machinery gene
H681_RS04965 (H681_04935)	pilB	1073445..1075148 (-)	1704	WP_041711736.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H681_RS04970 (H681_04940)	-	1075374..1075835 (+)	462	WP_015475740.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31858.78 Da
• Isoelectric Point: 7.3228

>NTDB_id=57449 H681_RS04955 WP_015475737.1 1071346..1072218(-) (pilD) [Pseudomonas sp. ATCC 13867]
MPVLNFLASTPLAFVLCALLIGLLVGSFLNVVIHRLPRMMQRDWQREAREALELPAGEPQPTYNLVLPNSQCPHCGHEIR
AWENIPVVSYLALGGKCSSCKTPISKRYPLIELACGALSAFIAWHYGFGWQAGAMLLLGWGLLAMSMIDVDHQLLPDALV
LPLLWLGLIANYFGLFTNLGDALWGAVFGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAMVGAWGGWQVLPLTILLSSLVG
AILGVITLRLRKAETGTPIPFGPYLAIAGWIALLWGDQITATYLQFAGFH

Nucleotide

• Length: 873 bp

>NTDB_id=57449 H681_RS04955 WP_015475737.1 1071346..1072218(-) (pilD) [Pseudomonas sp. ATCC 13867]
ATGCCAGTCCTGAACTTCCTGGCCAGCACCCCGCTGGCCTTTGTTTTATGCGCCCTGCTGATCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTGGTCATCCACCGCCTGCCGCGGATGATGCAGCGCGACTGGCAACGCGAGGCGCGCGAAGCGCTGGAGC
TGCCCGCAGGCGAGCCGCAGCCCACCTACAACCTGGTTCTGCCGAACTCCCAGTGCCCGCATTGCGGCCACGAGATCAGG
GCCTGGGAAAACATCCCGGTGGTGAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGACGCCCATCAGCAAGCG
CTATCCGCTGATCGAACTGGCCTGTGGCGCGCTCTCGGCCTTCATCGCCTGGCACTACGGTTTCGGCTGGCAGGCCGGCG
CCATGCTGCTGCTTGGCTGGGGTCTGCTGGCGATGAGCATGATCGACGTCGATCACCAGTTGCTGCCGGATGCGCTGGTG
CTGCCGTTGCTGTGGCTGGGACTGATCGCCAACTACTTCGGGCTGTTCACCAACCTGGGTGATGCGCTCTGGGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGCTCACCGGCAAGGAAGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCGATGGTCGGCGCCTGGGGTGGCTGGCAGGTGCTGCCGCTGACCATTCTGCTGTCGTCGCTGGTGGGC
GCCATTCTCGGCGTAATCACCCTGCGCCTGCGCAAGGCCGAAACCGGCACGCCGATCCCCTTCGGGCCCTATCTCGCCAT
TGCCGGCTGGATTGCGCTGCTCTGGGGTGATCAAATAACCGCGACTTATCTGCAGTTTGCCGGTTTCCACTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: M4XC34

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.797	95.172	0.531
pilD	Vibrio campbellii strain DS40M4	54.348	95.172	0.517
pilD	Acinetobacter nosocomialis M2	49.648	97.931	0.486
pilD	Acinetobacter baumannii D1279779	49.648	97.931	0.486
pilD	Neisseria gonorrhoeae MS11	51.292	93.448	0.479