Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KM146_RS19715 Genome accession   NZ_CP076287
Coordinates   2549659..2550252 (-) Length   197 a.a.
NCBI ID   WP_042523672.1    Uniprot ID   A0A7Y0R197
Organism   Vibrio alginolyticus strain ZJ-0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544659..2555252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM146_RS19695 (KM146_19690) aroB 2545544..2546644 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  KM146_RS19700 (KM146_19695) aroK 2546677..2547195 (-) 519 WP_005381319.1 shikimate kinase AroK -
  KM146_RS19705 (KM146_19700) pilQ 2547383..2549128 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  KM146_RS19710 (KM146_19705) pilP 2549154..2549669 (-) 516 WP_065274915.1 pilus assembly protein PilP Machinery gene
  KM146_RS19715 (KM146_19710) pilO 2549659..2550252 (-) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  KM146_RS19720 (KM146_19715) pilN 2550245..2550820 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  KM146_RS19725 (KM146_19720) pilM 2550804..2551820 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  KM146_RS19730 (KM146_19725) - 2551965..2554526 (+) 2562 WP_065274916.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22999.53 Da        Isoelectric Point: 5.0948

>NTDB_id=574419 KM146_RS19715 WP_042523672.1 2549659..2550252(-) (pilO) [Vibrio alginolyticus strain ZJ-0]
MVDWQDLEIDEMAEWPLLPQIVVIVLLIMAIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEAKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=574419 KM146_RS19715 WP_042523672.1 2549659..2550252(-) (pilO) [Vibrio alginolyticus strain ZJ-0]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGTACTTCT
GATCATGGCGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATTCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTTC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGTGCTAATACCTATCA
GTTTAAGCCGGAGGAGGCGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0R197

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685