Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   KM146_RS19720 Genome accession   NZ_CP076287
Coordinates   2550245..2550820 (-) Length   191 a.a.
NCBI ID   WP_054577259.1    Uniprot ID   A0AAW6ZZY0
Organism   Vibrio alginolyticus strain ZJ-0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2545245..2555820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM146_RS19695 (KM146_19690) aroB 2545544..2546644 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  KM146_RS19700 (KM146_19695) aroK 2546677..2547195 (-) 519 WP_005381319.1 shikimate kinase AroK -
  KM146_RS19705 (KM146_19700) pilQ 2547383..2549128 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  KM146_RS19710 (KM146_19705) pilP 2549154..2549669 (-) 516 WP_065274915.1 pilus assembly protein PilP Machinery gene
  KM146_RS19715 (KM146_19710) pilO 2549659..2550252 (-) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  KM146_RS19720 (KM146_19715) pilN 2550245..2550820 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  KM146_RS19725 (KM146_19720) pilM 2550804..2551820 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  KM146_RS19730 (KM146_19725) - 2551965..2554526 (+) 2562 WP_065274916.1 penicillin-binding protein 1A -
  KM146_RS19735 (KM146_19730) oxyR 2554581..2555492 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22362.86 Da        Isoelectric Point: 7.4657

>NTDB_id=574420 KM146_RS19720 WP_054577259.1 2550245..2550820(-) (pilN) [Vibrio alginolyticus strain ZJ-0]
MLYRINLLPWRENQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQDRLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=574420 KM146_RS19720 WP_054577259.1 2550245..2550820(-) (pilN) [Vibrio alginolyticus strain ZJ-0]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAATCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGATC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCACAGC
AAAATCCTCGAACGCCTAAAGGTTGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCTGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.723

100

0.827
  pilN Vibrio cholerae strain A1552

62.712

92.67

0.581