Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   CPY99_RS06850 Genome accession   NZ_AP017620
Coordinates   1333853..1334863 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain MRY15-131     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1328853..1339863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPY99_RS06820 (MRY15131_c12440) nudB 1330577..1331029 (+) 453 WP_077249130.1 dihydroneopterin triphosphate diphosphatase -
  CPY99_RS06825 (MRY15131_c12450) yebC 1331058..1331798 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CPY99_RS06830 (MRY15131_c12460) ruvC 1331833..1332354 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  CPY99_RS06835 (MRY15131_c12470) yebB 1332356..1332958 (-) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  CPY99_RS06840 yobI 1333029..1333094 (+) 66 WP_072127750.1 stress response small protein YobI -
  CPY99_RS06845 (MRY15131_c12480) ruvA 1333233..1333844 (+) 612 WP_061092987.1 Holliday junction branch migration protein RuvA -
  CPY99_RS06850 (MRY15131_c12490) ruvB 1333853..1334863 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  CPY99_RS06860 (MRY15131_c12500) - 1335130..1336110 (+) 981 WP_000399664.1 IS110-like element IS621 family transposase -
  CPY99_RS06865 (MRY15131_c12510) znuB 1336287..1337072 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  CPY99_RS06870 (MRY15131_c12520) znuC 1337069..1337824 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  CPY99_RS06875 (MRY15131_c12530) znuA 1337903..1338835 (+) 933 WP_024179478.1 zinc ABC transporter substrate-binding protein ZnuA -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=57225 CPY99_RS06850 WP_000568519.1 1333853..1334863(+) (ruvB) [Escherichia coli strain MRY15-131]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=57225 CPY99_RS06850 WP_000568519.1 1333853..1334863(+) (ruvB) [Escherichia coli strain MRY15-131]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACGTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAACCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCCGGCGATCTGGCAGCGATGCTCACTAATCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACACGTGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGACTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment