NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	KM132_RS12300	Genome accession	NZ_CP076119
Coordinates	2544237..2545307 (-)	Length	356 a.a.
NCBI ID	WP_154817565.1	Uniprot ID	-
Organism	Bacillus velezensis strain NZ4
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2539237..2550307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KM132_RS12250 (KM132_12250)	sipW	2539296..2539880 (-)	585	WP_012117977.1	signal peptidase I SipW	-
KM132_RS12255 (KM132_12255)	tapA	2539852..2540523 (-)	672	WP_014418371.1	amyloid fiber anchoring/assembly protein TapA	-
KM132_RS12260 (KM132_12260)	-	2540782..2541111 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
KM132_RS12265 (KM132_12265)	-	2541152..2541331 (-)	180	WP_003153093.1	YqzE family protein	-
KM132_RS12270 (KM132_12270)	comGG	2541388..2541765 (-)	378	WP_017418138.1	competence type IV pilus minor pilin ComGG	Machinery gene
KM132_RS12275 (KM132_12275)	comGF	2541766..2542161 (-)	396	WP_154817562.1	competence type IV pilus minor pilin ComGF	-
KM132_RS12280 (KM132_12280)	comGE	2542175..2542489 (-)	315	WP_021494312.1	competence type IV pilus minor pilin ComGE	Machinery gene
KM132_RS12285 (KM132_12285)	comGD	2542473..2542910 (-)	438	WP_154817563.1	competence type IV pilus minor pilin ComGD	Machinery gene
KM132_RS12290 (KM132_12290)	comGC	2542900..2543208 (-)	309	WP_014418376.1	competence type IV pilus major pilin ComGC	Machinery gene
KM132_RS12295 (KM132_12295)	comGB	2543213..2544250 (-)	1038	WP_154817564.1	competence type IV pilus assembly protein ComGB	Machinery gene
KM132_RS12300 (KM132_12300)	comGA	2544237..2545307 (-)	1071	WP_154817565.1	competence type IV pilus ATPase ComGA	Machinery gene
KM132_RS12305 (KM132_12305)	-	2545500..2546450 (-)	951	WP_014418379.1	magnesium transporter CorA family protein	-
KM132_RS12310 (KM132_12310)	-	2546596..2547897 (+)	1302	WP_029973873.1	hemolysin family protein	-
KM132_RS12315 (KM132_12315)	-	2547935..2548765 (-)	831	WP_154817566.1	STAS domain-containing protein	-
KM132_RS12325 (KM132_12325)	-	2548972..2549352 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
KM132_RS12330 (KM132_12330)	-	2549589..2549834 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40571.89 Da Isoelectric Point: 9.4784

                                >NTDB_id=572118 KM132_RS12300 WP_154817565.1 2544237..2545307(-) (comGA) [Bacillus velezensis strain NZ4]

MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLINY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=572118 KM132_RS12300 WP_154817565.1 2544237..2545307(-) (comGA) [Bacillus velezensis strain NZ4]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG

TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTATCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGATCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGTGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0572118