Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   KM132_RS12285 Genome accession   NZ_CP076119
Coordinates   2542473..2542910 (-) Length   145 a.a.
NCBI ID   WP_154817563.1    Uniprot ID   -
Organism   Bacillus velezensis strain NZ4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2537473..2547910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM132_RS12235 (KM132_12235) sinI 2537856..2538029 (+) 174 WP_014418369.1 anti-repressor SinI Regulator
  KM132_RS12240 (KM132_12240) sinR 2538063..2538398 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  KM132_RS12245 (KM132_12245) tasA 2538446..2539231 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  KM132_RS12250 (KM132_12250) sipW 2539296..2539880 (-) 585 WP_012117977.1 signal peptidase I SipW -
  KM132_RS12255 (KM132_12255) tapA 2539852..2540523 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  KM132_RS12260 (KM132_12260) - 2540782..2541111 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  KM132_RS12265 (KM132_12265) - 2541152..2541331 (-) 180 WP_003153093.1 YqzE family protein -
  KM132_RS12270 (KM132_12270) comGG 2541388..2541765 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  KM132_RS12275 (KM132_12275) comGF 2541766..2542161 (-) 396 WP_154817562.1 competence type IV pilus minor pilin ComGF -
  KM132_RS12280 (KM132_12280) comGE 2542175..2542489 (-) 315 WP_021494312.1 competence type IV pilus minor pilin ComGE Machinery gene
  KM132_RS12285 (KM132_12285) comGD 2542473..2542910 (-) 438 WP_154817563.1 competence type IV pilus minor pilin ComGD Machinery gene
  KM132_RS12290 (KM132_12290) comGC 2542900..2543208 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  KM132_RS12295 (KM132_12295) comGB 2543213..2544250 (-) 1038 WP_154817564.1 competence type IV pilus assembly protein ComGB Machinery gene
  KM132_RS12300 (KM132_12300) comGA 2544237..2545307 (-) 1071 WP_154817565.1 competence type IV pilus ATPase ComGA Machinery gene
  KM132_RS12305 (KM132_12305) - 2545500..2546450 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  KM132_RS12310 (KM132_12310) - 2546596..2547897 (+) 1302 WP_029973873.1 hemolysin family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16226.74 Da        Isoelectric Point: 10.1554

>NTDB_id=572115 KM132_RS12285 WP_154817563.1 2542473..2542910(-) (comGD) [Bacillus velezensis strain NZ4]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGKIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=572115 KM132_RS12285 WP_154817563.1 2542473..2542910(-) (comGD) [Bacillus velezensis strain NZ4]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAAGATTGTCGAGCGTTCTTTTGATTCGCTTCATATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATAACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

55.479

100

0.559