Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   KMZ31_RS19405 Genome accession   NZ_CP076117
Coordinates   3912746..3913264 (-) Length   172 a.a.
NCBI ID   WP_007615071.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain CQN-2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3896514..3913591 3912746..3913264 within 0


Gene organization within MGE regions


Location: 3896514..3913591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ31_RS19315 (KMZ31_19175) - 3896514..3897422 (-) 909 WP_032875365.1 LysR family transcriptional regulator -
  KMZ31_RS19320 (KMZ31_19180) - 3897541..3898326 (+) 786 WP_032875362.1 3-hydroxybutyrate dehydrogenase -
  KMZ31_RS19325 (KMZ31_19185) - 3898511..3899959 (-) 1449 WP_032875359.1 PLP-dependent aminotransferase family protein -
  KMZ31_RS19330 (KMZ31_19190) - 3900088..3900999 (+) 912 WP_032875356.1 EamA family transporter -
  KMZ31_RS19335 (KMZ31_19195) - 3901150..3901320 (+) 171 Protein_3758 DUF255 domain-containing protein -
  KMZ31_RS19340 - 3901488..3901964 (+) 477 WP_023357232.1 hypothetical protein -
  KMZ31_RS19345 (KMZ31_19205) - 3902052..3902399 (+) 348 WP_032876887.1 hypothetical protein -
  KMZ31_RS19350 - 3902818..3902922 (-) 105 WP_229023903.1 hypothetical protein -
  KMZ31_RS19355 (KMZ31_19215) - 3903224..3903805 (-) 582 WP_032875349.1 hypothetical protein -
  KMZ31_RS19360 (KMZ31_19220) - 3904407..3906476 (+) 2070 WP_032875346.1 thioredoxin domain-containing protein -
  KMZ31_RS19365 (KMZ31_19225) - 3906473..3907369 (-) 897 WP_032875344.1 CPBP family intramembrane glutamic endopeptidase -
  KMZ31_RS19370 (KMZ31_19230) - 3907584..3908915 (+) 1332 WP_032875341.1 MFS transporter -
  KMZ31_RS19375 (KMZ31_19235) - 3908947..3909327 (-) 381 WP_032875338.1 VOC family protein -
  KMZ31_RS19380 (KMZ31_19240) - 3909384..3910322 (-) 939 WP_032875335.1 LacI family DNA-binding transcriptional regulator -
  KMZ31_RS19385 (KMZ31_19245) xth 3910385..3911143 (-) 759 WP_032875332.1 exodeoxyribonuclease III -
  KMZ31_RS19390 (KMZ31_19250) - 3911140..3911697 (-) 558 WP_032875329.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  KMZ31_RS19395 (KMZ31_19255) - 3911694..3912275 (-) 582 WP_032875326.1 bifunctional transcriptional activator/DNA repair enzyme AdaA -
  KMZ31_RS19400 (KMZ31_19260) rpsR 3912463..3912702 (-) 240 WP_029325793.1 30S ribosomal protein S18 -
  KMZ31_RS19405 (KMZ31_19265) ssbA 3912746..3913264 (-) 519 WP_007615071.1 single-stranded DNA-binding protein SsbA Machinery gene
  KMZ31_RS19410 (KMZ31_19270) rpsF 3913304..3913591 (-) 288 WP_004392977.1 30S ribosomal protein S6 -

Sequence


Protein


Download         Length: 172 a.a.        Molecular weight: 18772.33 Da        Isoelectric Point: 4.7621

>NTDB_id=572071 KMZ31_RS19405 WP_007615071.1 3912746..3913264(-) (ssbA) [Bacillus amyloliquefaciens strain CQN-2]
MLNRVVLVGRLTKDPELRYTPSGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNNGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=572071 KMZ31_RS19405 WP_007615071.1 3912746..3913264(-) (ssbA) [Bacillus amyloliquefaciens strain CQN-2]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAGCGGTGCGGCTGTCGC
CACGTTTACTCTTGCTGTGAATCGTACATTCACGAACCAGTCCGGAGAACGTGAAGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTTCTTAAAAAAGGAAGCCTTGCCGGCGTAGACGGCCGACTCCAGACAAGA
AATTATGAAAACCAGCAAGGACAGCGTGTCTTCGTGACAGAAGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCTAAAAA
CAACGGCGGTTCTGGATCAGGCGGATACAACGAAGGAAACAGCGGCGGAGGCCAATACTTTGGCGGAGGCCAAAATGATA
ATCCGTTCGGCGGAAATCAGAACAACCAGAGACGAAATCAAGGGAACAGCTTTAATGATGATCCATTTGCCAACGACGGC
AAGCCGATTGACATCTCGGATGATGATCTTCCGTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

98.837

100

0.988

  ssb Latilactobacillus sakei subsp. sakei 23K

58.757

100

0.605

  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395

  ssb Glaesserella parasuis strain SC1401

34.973

100

0.372