Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R2APBS1_RS16235 Genome accession   NC_020541
Coordinates   3533899..3534957 (-) Length   352 a.a.
NCBI ID   WP_007509502.1    Uniprot ID   I4WVW8
Organism   Rhodanobacter denitrificans     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3528899..3539957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2APBS1_RS16215 (R2APBS1_3340) - 3529782..3531926 (-) 2145 WP_041676810.1 type IV pilus secretin PilQ -
  R2APBS1_RS16220 (R2APBS1_3341) - 3531982..3532524 (-) 543 WP_015448756.1 pilus assembly protein PilP -
  R2APBS1_RS16225 (R2APBS1_3342) pilO 3532524..3533177 (-) 654 WP_007509498.1 type 4a pilus biogenesis protein PilO -
  R2APBS1_RS16230 (R2APBS1_3343) - 3533174..3533899 (-) 726 WP_015448757.1 PilN domain-containing protein -
  R2APBS1_RS16235 (R2APBS1_3344) pilM 3533899..3534957 (-) 1059 WP_007509502.1 pilus assembly protein PilM Machinery gene
  R2APBS1_RS16240 (R2APBS1_3345) - 3535230..3537758 (+) 2529 WP_015448758.1 penicillin-binding protein 1A -
  R2APBS1_RS16245 (R2APBS1_3346) - 3537820..3538491 (+) 672 WP_015448759.1 hypothetical protein -
  R2APBS1_RS16250 (R2APBS1_3347) - 3538562..3539857 (-) 1296 WP_015448760.1 citrate synthase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37848.39 Da        Isoelectric Point: 4.5116

>NTDB_id=57139 R2APBS1_RS16235 WP_007509502.1 3533899..3534957(-) (pilM) [Rhodanobacter denitrificans]
MGLFTPKKPALIGVDISSTAVKLLQLSQAGGRYRVEHYAVEPLPPNAVVEKNIVEIEAVGDAIRRALARSGSKLKHAAAA
VAGSAVITRIIPMTGDLSDDDLEGQIQVEANQYIPYPIDEVSLDYEVLGPVRDNPDMNNVLLAASRTENVDMRVAALDLG
GLTAAVIDVEAFAMENAFAMIADQLNVGRDALVAVVDIGATMTTLSVLRNQRTIYSREQVFGGKQLTDEIMRRFGLSYEE
AGRAKRKGGLPESYETEALEPFKESLIQQISRLLQFFFAGSEYSKVDQVVLAGGCASIEGLGQMLEEQLGVPCVVANPLA
RMSLSPRVQAQSLAQDAPALMIAVGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=57139 R2APBS1_RS16235 WP_007509502.1 3533899..3534957(-) (pilM) [Rhodanobacter denitrificans]
GTGGGGCTCTTTACACCGAAGAAGCCGGCGCTGATTGGTGTCGACATCAGTTCGACTGCCGTCAAGCTGCTGCAGTTAAG
TCAGGCGGGCGGCCGTTATCGCGTTGAGCATTACGCGGTCGAGCCGTTGCCGCCGAACGCCGTGGTCGAAAAGAACATCG
TCGAGATCGAGGCGGTGGGCGATGCGATCCGCCGCGCGCTGGCACGCTCGGGCTCCAAGCTCAAGCACGCCGCCGCCGCG
GTGGCCGGGTCGGCCGTGATCACGCGCATCATTCCGATGACCGGCGATCTTTCCGATGACGACCTGGAAGGCCAGATCCA
GGTCGAGGCGAACCAGTACATTCCGTACCCGATCGACGAGGTCAGCCTCGACTACGAGGTGCTCGGTCCGGTCCGCGACA
ACCCCGACATGAACAACGTGCTGCTGGCCGCCTCGCGCACCGAGAACGTCGACATGCGCGTGGCCGCGCTGGACCTGGGC
GGGCTCACCGCCGCGGTGATCGACGTCGAGGCGTTCGCGATGGAGAACGCTTTCGCGATGATCGCCGACCAGCTCAACGT
GGGCCGTGACGCGTTGGTGGCGGTGGTCGACATCGGTGCCACCATGACCACGCTGTCGGTGCTGCGCAACCAGCGCACGA
TCTACTCGCGCGAACAGGTGTTCGGCGGCAAGCAGCTGACCGACGAGATCATGCGTCGCTTCGGGCTGTCCTACGAGGAA
GCCGGCCGCGCCAAGCGCAAGGGCGGCCTGCCCGAGTCCTACGAGACCGAGGCGCTGGAGCCGTTCAAGGAATCGCTGAT
CCAGCAGATCAGCCGGCTGCTGCAGTTCTTCTTTGCCGGCAGCGAGTACAGCAAGGTCGACCAGGTGGTGCTGGCCGGCG
GTTGTGCCTCGATCGAGGGGCTCGGCCAGATGCTGGAAGAGCAGCTCGGCGTGCCCTGCGTCGTCGCCAATCCGTTGGCC
CGCATGTCGCTGTCGCCGCGGGTCCAGGCACAGTCGCTGGCGCAGGACGCCCCCGCGTTGATGATTGCGGTCGGCCTCGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4WVW8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.273

100

0.523
  comM Acinetobacter nosocomialis M2

51.989

100

0.52
  comM Acinetobacter baylyi ADP1

51.714

99.432

0.514
  pilM Legionella pneumophila strain ERS1305867

43.059

100

0.432

Multiple sequence alignment