Detailed information NTG0057127

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R2APBS1_RS04140
• Genome accession: NC_020541
• Coordinates: 946480..947646 (+)
• Length: 388 a.a.
• NCBI ID: WP_015446994.1
• Uniprot ID: -
• Organism: Rhodanobacter denitrificans
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 941480..952646

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R2APBS1_RS04120 (R2APBS1_0845)	-	941860..942684 (+)	825	WP_015446992.1	ABC transporter substrate-binding protein	-
R2APBS1_RS04125 (R2APBS1_0846)	-	943524..944099 (-)	576	WP_007507063.1	YggT family protein	-
R2APBS1_RS04130 (R2APBS1_0847)	proC	944096..944920 (-)	825	WP_015446993.1	pyrroline-5-carboxylate reductase	-
R2APBS1_RS04135 (R2APBS1_0848)	pilT	945289..946326 (+)	1038	WP_007507061.1	type IV pilus twitching motility protein PilT	Machinery gene
R2APBS1_RS04140 (R2APBS1_0849)	pilU	946480..947646 (+)	1167	WP_015446994.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R2APBS1_RS04145 (R2APBS1_0850)	-	947713..948090 (-)	378	WP_015446995.1	BON domain-containing protein	-
R2APBS1_RS04150 (R2APBS1_0851)	-	948443..950653 (-)	2211	WP_236127000.1	TonB-dependent siderophore receptor	-
R2APBS1_RS04155 (R2APBS1_0852)	-	950799..951491 (+)	693	WP_015446997.1	response regulator transcription factor	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43408.83 Da
• Isoelectric Point: 6.7735

>NTDB_id=57127 R2APBS1_RS04140 WP_015446994.1 946480..947646(+) (pilU) [Rhodanobacter denitrificans]
MSDFDFTSFLKLMVHKKASDLFITAGVAPSMKVQGRIVPITQSPLSVQQARDMVLNVMTPGQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIESKIPTIEELTLPPVIKQLAMTKRGIIVFVGATGSGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQREVGIDTDSWDNALKNTLRQAPDVIMIGEIRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRILHFFPEDRRQQLLMDLSLNLKGIVAQQLIPTPDGKARRVAVEILLGTPLVQDYIRQGEIHKLKEVMKESTLLGMKTF
DQSLVELYHAGEISYEDALRYADSSNEVRLRIKLAQGGDAHTLSQGLEGVELEESRDAQNFSSNFLNR

Nucleotide

• Length: 1167 bp

>NTDB_id=57127 R2APBS1_RS04140 WP_015446994.1 946480..947646(+) (pilU) [Rhodanobacter denitrificans]
ATGAGCGACTTCGATTTCACCTCATTCCTGAAGCTGATGGTGCACAAGAAGGCCTCCGACTTGTTCATCACGGCGGGCGT
CGCCCCCTCGATGAAAGTGCAGGGCCGGATCGTGCCGATCACGCAGAGCCCGCTCAGTGTGCAGCAGGCGCGCGACATGG
TGCTCAACGTGATGACGCCGGGCCAGCGCGAGGAGTTCGAGAAGACCCATGAGTGCCAGTTCGCGATCTCCGCGCAAGGC
GTGGGCCGTTTCCGCGTGTCGTGCTTCTACCAGCGCAACTGCGTGGGTATGGTGCTGCGCCGGATCGAGTCGAAGATCCC
CACCATCGAGGAACTGACGCTGCCGCCGGTGATCAAGCAACTGGCGATGACCAAGCGCGGCATCATCGTGTTCGTCGGCG
CCACCGGCTCGGGCAAATCGACCTCGCTGGCGTCGATGATCGGCTACCGCAACCAGAACTCGACCGGTCATATCATCACG
ATCGAGGACCCGATCGAGTACGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGACAACGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAAATCCGCACCCGCGAGGGCA
TGGACCACGCGATCGCCTTTGCCGAAACCGGTCACCTGGTGCTGTGCACGCTGCACGCGAACAACGCCAACCAGGCGATG
GACCGCATCCTGCACTTCTTCCCCGAGGACCGCCGCCAGCAACTGCTGATGGACCTGTCGCTCAACCTGAAGGGCATCGT
GGCGCAGCAGCTGATCCCCACGCCCGACGGCAAGGCGCGCCGGGTCGCGGTGGAGATTCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCCAGGGCGAGATCCACAAGCTGAAGGAGGTGATGAAGGAGTCCACTCTGCTCGGCATGAAGACCTTC
GACCAGAGCCTGGTCGAGCTGTACCACGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACAGCTCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCATACCCTGTCGCAAGGCCTGGAAGGCGTCGAGCTGGAGG
AAAGCCGCGACGCGCAGAACTTCAGCAGCAATTTCCTCAACCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.578	96.392	0.642
pilU	Acinetobacter baylyi ADP1	63.483	91.753	0.582
pilU	Vibrio cholerae strain A1552	52.809	91.753	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.129	95.876	0.394