Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C4C37_RS02230 Genome accession   NZ_CP075686
Coordinates   493024..494088 (+) Length   354 a.a.
NCBI ID   WP_002551652.1    Uniprot ID   A0A7Z6Y2Y4
Organism   Pseudomonas amygdali pv. lachrymans strain 8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488024..499088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4C37_RS02220 (C4C37_02220) - 488361..489629 (+) 1269 WP_002551650.1 malic enzyme-like NAD(P)-binding protein -
  C4C37_RS02225 (C4C37_02225) - 490373..492814 (-) 2442 WP_371911680.1 penicillin-binding protein 1A -
  C4C37_RS02230 (C4C37_02230) pilM 493024..494088 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  C4C37_RS02235 (C4C37_02235) - 494088..494687 (+) 600 WP_004654254.1 PilN domain-containing protein -
  C4C37_RS02240 (C4C37_02240) pilO 494684..495307 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  C4C37_RS02245 (C4C37_02245) pilP 495304..495831 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  C4C37_RS02250 (C4C37_02250) pilQ 495879..497984 (+) 2106 WP_005743117.1 type IV pilus secretin PilQ Machinery gene
  C4C37_RS02255 (C4C37_02255) aroK 497989..498507 (+) 519 WP_002551657.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38078.57 Da        Isoelectric Point: 4.7008

>NTDB_id=570173 C4C37_RS02230 WP_002551652.1 493024..494088(+) (pilM) [Pseudomonas amygdali pv. lachrymans strain 8]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=570173 C4C37_RS02230 WP_002551652.1 493024..494088(+) (pilM) [Pseudomonas amygdali pv. lachrymans strain 8]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGCCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGATGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAACGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GAATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGCCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGATTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCCCGTTCTTTGCAGTTCTTCTTTGCAGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCTAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z6Y2Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469