Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KI610_RS19705 Genome accession   NZ_CP075189
Coordinates   4096048..4097520 (-) Length   490 a.a.
NCBI ID   WP_226496631.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 77     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4091048..4102520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI610_RS19675 (KI610_19585) ttcA 4092075..4092938 (+) 864 WP_226496627.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  KI610_RS19680 (KI610_19590) - 4093031..4093903 (+) 873 WP_226498585.1 adenylate/guanylate cyclase domain-containing protein -
  KI610_RS19685 (KI610_19595) - 4093953..4094210 (-) 258 WP_226496628.1 hypothetical protein -
  KI610_RS19690 (KI610_19600) hprK 4094258..4095193 (-) 936 WP_226496629.1 HPr(Ser) kinase/phosphatase -
  KI610_RS19695 (KI610_19605) ptsN 4095203..4095649 (-) 447 WP_226496630.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KI610_RS19700 (KI610_19610) hpf 4095706..4096032 (-) 327 WP_226403128.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KI610_RS19705 (KI610_19615) rpoN/rpoN1 4096048..4097520 (-) 1473 WP_226496631.1 RNA polymerase factor sigma-54 Machinery gene
  KI610_RS19710 (KI610_19620) lptB 4097548..4098282 (-) 735 WP_404827441.1 LPS export ABC transporter ATP-binding protein -
  KI610_RS19715 (KI610_19625) recX 4098279..4098710 (-) 432 WP_226496632.1 recombination regulator RecX -
  KI610_RS19720 (KI610_19630) recA 4098703..4099731 (-) 1029 WP_226496633.1 recombinase RecA Machinery gene
  KI610_RS19725 (KI610_19635) - 4099790..4100170 (+) 381 WP_226496634.1 hypothetical protein -
  KI610_RS19730 (KI610_19640) - 4100164..4100820 (+) 657 WP_226498587.1 pseudouridine synthase -
  KI610_RS19735 (KI610_19645) - 4100891..4102432 (+) 1542 WP_226496635.1 sodium:solute symporter family protein -

Sequence

Protein


Download         Length: 490 a.a.        Molecular weight: 55440.58 Da        Isoelectric Point: 5.1404

>NTDB_id=568542 KI610_RS19705 WP_226496631.1 4096048..4097520(-) (rpoN/rpoN1) [Ferribacterium limneticum strain 77]
MKPALQLKLSQHLALTPQLQQSIKLLQLSTIEMQQEIERYLLENPMLEREDEQGGEAFSSAQQFDSPQTSEGEREQKAER
EERDARDDREQDVPSAPSDVDDDRWAADAGTFTGAGRDEDDDSDSQDIHGTTISLRDHLGWQLGMTQVSERDRSLVRFLI
EALDDDGYLSATLEELWETLPPEYEIEIEELEIALHHIQNFDPVGIGARNLQECLALQLKALPGSCEVRDLALTIVQKHL
ELLAARDFAKIRRLTGCDDESLKAAQSVITGLNPRPASRFSQIEARYITPDVIVKKLKGKWTAYINPDAYPRLRINRLYA
EILSKQRRGNGHLSTQLQEARWLIKNVQQRFETIHRVTQAIVDRQRQFFEHGEVAMRPLVLREIADILGLHESTVSRVTS
QKYMATPRGIFELKYFFGSHVSTDTGGACSATAIRALIKQLISAEDGKKPLSDSQLSEILGQQGIVVARRTVAKYRESLN
IPPVNLRKTL

Nucleotide


Download         Length: 1473 bp        

>NTDB_id=568542 KI610_RS19705 WP_226496631.1 4096048..4097520(-) (rpoN/rpoN1) [Ferribacterium limneticum strain 77]
ATGAAGCCGGCACTCCAGCTAAAACTCTCCCAACATTTGGCGCTGACGCCTCAGCTGCAGCAGTCAATCAAACTGCTACA
GCTGTCGACAATCGAAATGCAGCAGGAAATCGAGCGTTATCTGCTCGAGAATCCAATGCTGGAGCGCGAGGACGAGCAGG
GCGGCGAGGCATTTTCCAGTGCTCAGCAATTCGACTCACCGCAAACCAGTGAGGGCGAGCGCGAACAAAAGGCTGAGCGC
GAAGAGCGCGATGCACGCGACGACCGCGAGCAGGACGTTCCGTCAGCTCCCTCCGATGTCGATGACGACCGTTGGGCCGC
CGATGCCGGCACCTTCACCGGGGCCGGGCGCGACGAAGACGACGACAGCGATTCGCAGGACATTCACGGCACGACGATCA
GCCTGCGCGATCATCTCGGCTGGCAACTGGGGATGACCCAGGTTTCGGAACGCGACCGAAGCCTGGTCCGTTTCCTGATT
GAGGCCCTCGATGACGATGGCTACCTGTCGGCTACCTTGGAAGAACTCTGGGAAACGCTGCCACCGGAATACGAAATTGA
AATCGAAGAACTGGAAATTGCGCTGCATCACATCCAGAATTTCGACCCGGTCGGCATTGGTGCCCGCAACCTCCAGGAAT
GCCTGGCCCTGCAACTCAAAGCCCTGCCGGGCAGTTGTGAGGTCCGCGATCTCGCGCTGACCATCGTCCAGAAGCACCTG
GAACTGCTGGCTGCCCGCGATTTCGCCAAGATCCGCCGCCTGACCGGCTGCGATGATGAGTCGCTGAAGGCCGCGCAATC
GGTGATTACCGGCCTGAATCCGCGACCGGCCTCACGCTTTTCCCAGATCGAAGCCCGCTACATCACGCCGGACGTGATCG
TAAAGAAGCTCAAGGGGAAGTGGACTGCGTACATCAATCCCGATGCTTATCCGCGCCTGCGCATCAACCGTCTTTACGCC
GAAATTCTTTCGAAGCAGCGGCGTGGCAATGGCCACCTGTCCACTCAGTTGCAGGAAGCTCGCTGGCTGATTAAAAATGT
TCAGCAGCGTTTTGAGACAATTCATCGTGTGACGCAGGCCATCGTTGATCGTCAGCGTCAGTTCTTTGAACACGGAGAAG
TTGCGATGCGCCCGCTGGTTTTGCGCGAAATCGCTGACATTCTCGGTTTGCACGAGTCGACCGTATCGCGCGTCACCAGC
CAGAAATACATGGCGACGCCGCGCGGTATCTTCGAACTCAAATACTTCTTCGGCAGTCATGTCTCCACCGATACCGGTGG
TGCCTGTTCTGCTACGGCCATCCGGGCGCTGATCAAGCAGTTGATCTCGGCCGAGGATGGCAAGAAGCCCTTGTCGGATA
GTCAATTATCCGAAATACTAGGCCAGCAGGGAATCGTAGTTGCCCGACGGACGGTTGCAAAATACCGCGAGTCGCTCAAC
ATCCCACCGGTCAATTTACGCAAGACCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(333-488)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-319)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.672

100

0.561