Detailed information NTG0056709

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AZKH_RS03635
• Genome accession: NC_020516
• Coordinates: 812560..813759 (+)
• Length: 399 a.a.
• NCBI ID: WP_015434384.1
• Uniprot ID: H0PX84
• Organism: Azoarcus sp. KH32C
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 807560..818759

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AZKH_RS03620 (AZKH_0734)	gmhB	809391..809930 (+)	540	WP_015434381.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
AZKH_RS03625 (AZKH_0735)	-	809939..810706 (+)	768	WP_015434382.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
AZKH_RS03630 (AZKH_0736)	-	810761..812449 (+)	1689	WP_015434383.1	sensor histidine kinase	-
AZKH_RS03635 (AZKH_0737)	pilU	812560..813759 (+)	1200	WP_015434384.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AZKH_RS03640 (AZKH_0738)	-	813907..814470 (-)	564	WP_015434385.1	phasin family protein	-
AZKH_RS03645 (AZKH_0739)	-	814881..815657 (-)	777	WP_015434386.1	enoyl-CoA hydratase	-
AZKH_RS03650 (AZKH_0740)	lptA	815682..816275 (-)	594	WP_015434387.1	lipopolysaccharide transport periplasmic protein LptA	-
AZKH_RS03655 (AZKH_0741)	lptC	816272..816853 (-)	582	WP_015434388.1	LPS export ABC transporter periplasmic protein LptC	-
AZKH_RS03660 (AZKH_0742)	-	816933..817928 (-)	996	WP_041655882.1	SIS domain-containing protein	-

Sequence

Protein

• Length: 399 a.a.
• Molecular weight: 44511.65 Da
• Isoelectric Point: 5.9442

>NTDB_id=56709 AZKH_RS03635 WP_015434384.1 812560..813759(+) (pilU) [Azoarcus sp. KH32C]
MSSGLSNEDARNYMHKLLKAMHQMGGSDLFISVDFPPSMKAHGTMKALSQQRLTSDVTRSLAYSLMNERQRTEFDAEFEC
NFAISLPDVCRFRVNVFIQQQNVGMVIRTIASEIPSIDKLGLPNVLREIIMTKRGLVLVVGGTGSGKSTTLAAMIDHRNS
NSPGHIITVEDPVEYVHKNKQCLITHREVGVDTHSWEHALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCLGTLH
ANNANQTIDRIVNFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGNGRKAAIEVLLNTPTISEMIFKGQFQSIKEIMAKS
RELGMCTFDQALFDLYNAGFISYEEAIRNADSANELRLQIKLKADRAQPTVESSGGLSLSIEEEKPDEDEEGAPDKAAV

Nucleotide

• Length: 1200 bp

>NTDB_id=56709 AZKH_RS03635 WP_015434384.1 812560..813759(+) (pilU) [Azoarcus sp. KH32C]
ATGTCGTCCGGACTGAGCAACGAAGACGCCCGCAACTACATGCACAAACTGCTCAAGGCGATGCACCAGATGGGCGGTTC
GGACCTCTTCATCTCAGTCGATTTTCCGCCCAGCATGAAGGCGCACGGCACCATGAAGGCGCTCAGTCAGCAACGCCTGA
CGTCCGACGTGACGCGCTCGCTCGCTTACAGCCTGATGAACGAACGCCAGCGCACCGAATTCGACGCGGAGTTCGAATGC
AACTTCGCGATTTCGCTGCCCGATGTCTGCCGCTTCCGCGTCAACGTGTTCATCCAGCAACAGAACGTGGGCATGGTGAT
CCGGACGATCGCTTCCGAGATCCCGAGCATCGACAAGCTCGGCCTGCCGAACGTGCTGCGCGAGATCATCATGACCAAGC
GCGGGCTGGTGTTGGTGGTCGGTGGTACCGGCTCCGGCAAGTCGACGACGCTTGCGGCGATGATCGATCACCGCAACAGC
AATTCGCCCGGCCACATCATCACGGTCGAGGATCCGGTCGAGTACGTGCACAAGAACAAGCAATGCCTGATCACGCACCG
CGAGGTGGGCGTCGACACGCACTCGTGGGAGCACGCCCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACCGCGAAACGATGGAGCACGCGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTCGGCACGCTGCAC
GCGAATAACGCCAACCAGACGATCGACCGCATCGTCAATTTCTTCCCCGAAGAGCGGCGCAACCAGCTGTTGATGGACTT
GTCCTCGAACCTGCGGGCGATCGTGTCACAGCGCCTGATTCGGACCGAAGACGGCAACGGGCGCAAGGCCGCGATCGAAG
TGCTGCTCAATACCCCGACGATCTCCGAGATGATCTTCAAGGGCCAGTTCCAGTCGATCAAGGAGATCATGGCCAAATCG
CGCGAACTCGGCATGTGCACCTTCGACCAGGCGCTCTTCGACCTTTATAACGCCGGATTCATCAGCTACGAGGAGGCGAT
CCGCAATGCCGATTCGGCCAACGAGCTGCGATTGCAGATCAAGCTCAAGGCCGATCGCGCGCAACCGACCGTCGAATCAT
CCGGCGGGCTGAGCCTTTCGATCGAGGAAGAGAAGCCGGACGAGGACGAGGAGGGTGCGCCGGACAAGGCGGCGGTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (88-282)

3D structure

• AlphaFold DB: H0PX84

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.452	93.233	0.526
pilU	Acinetobacter baylyi ADP1	55.65	88.722	0.494
pilU	Vibrio cholerae strain A1552	50.857	87.719	0.446
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.82	89.223	0.391