Detailed information NTG0056466

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LILO_RS05175
• Genome accession: NC_020450
• Coordinates: 1055373..1056695 (-)
• Length: 440 a.a.
• NCBI ID: WP_015426303.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis IO-1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1050373..1061695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LILO_RS05145 (lilo_0986)	-	1051517..1052485 (+)	969	WP_003130649.1	PhoH family protein	-
LILO_RS05150 (lilo_0987)	-	1052543..1053022 (+)	480	WP_003130650.1	NUDIX hydrolase	-
LILO_RS05155 (lilo_0988)	ybeY	1053174..1053662 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
LILO_RS05160 (lilo_0989)	-	1053646..1054101 (+)	456	WP_003130653.1	diacylglycerol kinase family protein	-
LILO_RS05165 (lilo_0990)	-	1054115..1054690 (+)	576	WP_015426301.1	nucleotidyltransferase family protein	-
LILO_RS12200	comFC	1054726..1055130 (-)	405	WP_225513299.1	ComF family protein	Machinery gene
LILO_RS05175 (lilo_0992)	comFA	1055373..1056695 (-)	1323	WP_015426303.1	DEAD/DEAH box helicase	Machinery gene
LILO_RS05180 (lilo_0993)	-	1056752..1057381 (+)	630	WP_015426304.1	YigZ family protein	-
LILO_RS05185 (lilo_0994)	-	1057515..1058012 (+)	498	WP_015426305.1	VanZ family protein	-
LILO_RS05190 (lilo_0995)	-	1058060..1059079 (+)	1020	WP_015426306.1	DUF475 domain-containing protein	-
LILO_RS05195 (lilo_0996)	-	1059194..1059940 (+)	747	WP_042230284.1	bifunctional metallophosphatase/5'-nucleotidase	-
LILO_RS05200 (lilo_0997)	glyQ	1060226..1061179 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50097.56 Da
• Isoelectric Point: 10.1468

>NTDB_id=56466 LILO_RS05175 WP_015426303.1 1055373..1056695(-) (comFA) [Lactococcus lactis subsp. lactis IO-1]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSKFLLETTVKSILGMTSISSNKIKCNRCGTVHIKNSVKLPIGAFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSVSNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKVEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=56466 LILO_RS05175 WP_015426303.1 1055373..1056695(-) (comFA) [Lactococcus lactis subsp. lactis IO-1]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAAATTTTTGTTAGAAACAACGGTAAAATCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAACTCTGTCAAACTTCCAATTGGTGCATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCGGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAGTTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTTCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAGGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAGTAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTTCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCACTTCTAATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAACTAAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-228)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74	90.909	0.673
comFA/cflA	Streptococcus mitis SK321	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae Rx1	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae D39	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae R6	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae TIGR4	52.513	90.455	0.475
comFA/cflA	Streptococcus mitis NCTC 12261	51.508	90.455	0.466
comFA	Bacillus subtilis subsp. subtilis str. 168	37.472	100	0.384