Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   KHW12_RS03235 Genome accession   NZ_CP074409
Coordinates   738166..739503 (-) Length   445 a.a.
NCBI ID   WP_003344575.1    Uniprot ID   A0A3M4Y9G0
Organism   Pseudomonas syringae strain KF529     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 733166..744503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW12_RS03220 (KHW12_03220) - 733288..736491 (+) 3204 WP_024664669.1 PilC/PilY family type IV pilus protein -
  KHW12_RS03225 (KHW12_03225) - 736503..736910 (+) 408 WP_003392819.1 type IV pilin protein -
  KHW12_RS03230 (KHW12_03230) thiO 736936..738039 (+) 1104 WP_003392818.1 glycine oxidase ThiO -
  KHW12_RS03235 (KHW12_03235) pilR 738166..739503 (-) 1338 WP_003344575.1 sigma-54 dependent transcriptional regulator Regulator
  KHW12_RS03240 (KHW12_03240) pilS 739506..741098 (-) 1593 WP_016567133.1 ATP-binding protein Regulator
  KHW12_RS03245 (KHW12_03245) - 741088..741327 (-) 240 WP_057445553.1 PP0621 family protein -
  KHW12_RS03250 (KHW12_03250) - 741448..742470 (-) 1023 WP_003344571.1 outer membrane protein assembly factor BamD -
  KHW12_RS03255 (KHW12_03255) rluD 742620..743582 (+) 963 WP_002551999.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  KHW12_RS03260 (KHW12_03260) pgeF 743579..744307 (+) 729 WP_032661019.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49669.60 Da        Isoelectric Point: 6.2986

>NTDB_id=564649 KHW12_RS03235 WP_003344575.1 738166..739503(-) (pilR) [Pseudomonas syringae strain KF529]
MSQRQKILIVDDEPDIRELLEITLGRMKLDTRSARNVAEAHDWLAREPFDMCLTDMRLPDGNGLELVQHIQHGYPHVPVA
MITAHGNVDTAINALKAGAFDFVTKPVDLGRLRELVNSALSLPGAPPTRSIDNRLLGDSLAMRTLRNQIGKLARSQAPIY
ISGESGSGKELVARLIHEQGPRIDKPFIPVNCGAIPSELMESEFFGHRKGSFSGAHEDKPGLFQAAHTGTLFLDEVADLP
LAMQVKLLRAIQEKSIRSVGGQQEQIVDVRILCATHKNLNAEVAAGRFRQDLYYRLNVIEVRVPSLRERREDIDQLAASV
LKRLAGNGAQPVARLNAQALETLKSYRFPGNVRELENMLERAYTLCENDEIHASDLRLTESASPQESDGPSLADIDNLED
YLESVERQLILQALEETRWNRTAAAERLSLSFRSLRYRLKKLGLD

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=564649 KHW12_RS03235 WP_003344575.1 738166..739503(-) (pilR) [Pseudomonas syringae strain KF529]
ATGAGCCAACGGCAAAAAATCCTGATAGTCGATGATGAGCCGGATATCCGCGAACTCCTGGAGATCACGCTGGGACGCAT
GAAACTCGACACCCGCAGTGCACGTAACGTTGCCGAAGCCCATGACTGGCTGGCCCGCGAACCGTTCGACATGTGCCTGA
CCGACATGCGTCTGCCTGATGGTAATGGCCTTGAGCTGGTGCAACACATACAGCACGGCTACCCCCACGTGCCGGTGGCG
ATGATCACTGCACACGGCAATGTGGATACCGCGATCAACGCGCTGAAGGCCGGTGCTTTCGATTTTGTGACCAAGCCGGT
CGACCTCGGCAGGCTGCGCGAGCTGGTCAATAGCGCGCTGAGCCTGCCCGGCGCACCACCCACGCGAAGCATCGACAACC
GACTGCTGGGCGACTCGCTGGCAATGCGTACCTTGCGTAATCAGATCGGCAAGCTGGCCCGCAGCCAGGCGCCGATCTAC
ATCAGCGGCGAGTCGGGCAGCGGCAAGGAGCTTGTAGCGCGTCTGATCCACGAGCAGGGCCCACGCATCGATAAACCTTT
CATACCGGTGAATTGCGGTGCCATCCCTTCCGAACTGATGGAGAGCGAATTCTTCGGTCATCGCAAAGGCAGTTTCAGCG
GTGCCCACGAAGACAAGCCCGGTCTGTTTCAAGCGGCGCACACCGGCACGCTGTTTCTCGATGAGGTCGCCGACCTGCCG
CTGGCCATGCAGGTCAAACTGCTGCGCGCCATCCAGGAAAAATCCATTCGCAGCGTCGGCGGCCAGCAAGAGCAGATCGT
CGACGTGCGCATTCTGTGCGCCACCCACAAGAACCTGAATGCAGAGGTCGCGGCAGGTCGGTTCCGGCAGGATCTGTATT
ACCGATTGAACGTGATCGAAGTACGCGTGCCCTCGTTGCGCGAGCGGCGTGAGGATATCGACCAACTGGCAGCCAGCGTG
CTCAAGCGCCTTGCCGGGAACGGCGCACAACCCGTCGCGCGCCTGAACGCGCAGGCGCTGGAAACGCTGAAGAGCTACCG
CTTCCCCGGTAACGTGCGCGAACTGGAAAACATGCTGGAGCGCGCTTACACGCTGTGCGAGAACGACGAAATCCACGCCA
GCGACCTGCGCCTGACAGAGTCAGCCAGCCCTCAGGAGAGCGACGGCCCGAGCCTGGCCGACATCGACAACCTTGAAGAC
TACCTGGAAAGCGTCGAACGCCAGCTCATTCTGCAGGCTCTGGAAGAAACCCGCTGGAACCGCACCGCCGCAGCAGAAAG
GCTCAGCTTGTCGTTCAGGTCACTGCGCTATCGCCTGAAGAAGCTGGGCCTGGATTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-116)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)

Sigma-54 interaction domain (Sigma54_activat)

(136-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M4Y9G0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

77.201

99.551

0.769
  pilR Acinetobacter baumannii strain A118

48.608

100

0.51
  luxO Vibrio cholerae strain A1552

37.613

99.775

0.375