Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   L6L57_RS18855 Genome accession   NZ_CP091712
Coordinates   3946274..3947767 (-) Length   497 a.a.
NCBI ID   WP_023141654.1    Uniprot ID   -
Organism   Escherichia coli strain KFu014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3941274..3952767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6L57_RS18825 (L6L57_18825) gspK 3942068..3943045 (-) 978 WP_000633200.1 type II secretion system minor pseudopilin GspK -
  L6L57_RS18830 (L6L57_18830) gspJ 3943042..3943647 (-) 606 WP_023147221.1 type II secretion system minor pseudopilin GspJ -
  L6L57_RS18835 (L6L57_18835) gspI 3943644..3944015 (-) 372 WP_000820109.1 type II secretion system minor pseudopilin GspI -
  L6L57_RS18840 (L6L57_18840) gspH 3944012..3944575 (-) 564 WP_001115147.1 type II secretion system minor pseudopilin GspH -
  L6L57_RS18845 (L6L57_18845) gspG 3944579..3945034 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  L6L57_RS18850 (L6L57_18850) gspF 3945051..3946274 (-) 1224 WP_001173427.1 type II secretion system inner membrane protein GspF -
  L6L57_RS18855 (L6L57_18855) pilB/pilB1 3946274..3947767 (-) 1494 WP_023141654.1 type II secretion system ATPase GspE Machinery gene
  L6L57_RS18860 (L6L57_18860) gspD 3947767..3949827 (-) 2061 WP_000498848.1 type II secretion system secretin GspD -
  L6L57_RS18865 (L6L57_18865) gspC 3949857..3950816 (-) 960 WP_023141653.1 type II secretion system protein GspC -
  L6L57_RS18870 (L6L57_18870) gspS2 3950834..3951244 (-) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  L6L57_RS18875 (L6L57_18875) pilD 3951310..3952119 (-) 810 WP_001296399.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55163.23 Da        Isoelectric Point: 5.9800

>NTDB_id=563307 L6L57_RS18855 WP_023141654.1 3946274..3947767(-) (pilB/pilB1) [Escherichia coli strain KFu014]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=563307 L6L57_RS18855 WP_023141654.1 3946274..3947767(-) (pilB/pilB1) [Escherichia coli strain KFu014]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
ACAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACACTGGAGCGTCTGGGTTTAAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTCACGGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTCTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGGCTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTTATGGGGATCTCCGATACGCATGCCGTCACGCTGTACCATCCGCAGGGTTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTACGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAGCGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Legionella pneumophila strain ERS1305867

41.97

93.964

0.394
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36