Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   KEF49_RS16965 Genome accession   NZ_CP073635
Coordinates   3341952..3343337 (-) Length   461 a.a.
NCBI ID   WP_212099490.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3336952..3348337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS16930 (KEF49_16930) flgL 3337126..3338043 (-) 918 WP_115996789.1 flagellar hook-associated protein FlgL -
  KEF49_RS16935 (KEF49_16935) flgK 3338055..3339572 (-) 1518 WP_065982450.1 flagellar hook-associated protein FlgK -
  KEF49_RS16940 (KEF49_16940) - 3339588..3340070 (-) 483 WP_013353780.1 flagellar protein FlgN -
  KEF49_RS16945 (KEF49_16945) flgM 3340085..3340351 (-) 267 WP_212099484.1 flagellar biosynthesis anti-sigma factor FlgM -
  KEF49_RS16950 (KEF49_16950) - 3340421..3340840 (-) 420 WP_212099487.1 TIGR03826 family flagellar region protein -
  KEF49_RS16955 (KEF49_16955) comFC 3340914..3341609 (-) 696 WP_231131128.1 double zinc ribbon domain-containing protein Machinery gene
  KEF49_RS16960 (KEF49_16960) - 3341609..3341893 (-) 285 WP_013353784.1 competence protein ComFB -
  KEF49_RS16965 (KEF49_16965) comFA 3341952..3343337 (-) 1386 WP_212099490.1 DEAD/DEAH box helicase Machinery gene
  KEF49_RS16970 (KEF49_16970) - 3343444..3344292 (-) 849 WP_212099493.1 DegV family protein -
  KEF49_RS16975 (KEF49_16975) degU 3344389..3345078 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  KEF49_RS16980 (KEF49_16980) degS 3345155..3346318 (-) 1164 WP_065982454.1 two-component sensor histidine kinase DegS Regulator
  KEF49_RS16985 (KEF49_16985) - 3346541..3347188 (+) 648 WP_212099496.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52634.13 Da        Isoelectric Point: 10.0235

>NTDB_id=559618 KEF49_RS16965 WP_212099490.1 3341952..3343337(-) (comFA) [Bacillus amyloliquefaciens strain Ba13]
MSGGVSEFSEVRDFFYGRHLLRSEIPFSDQCIKRFIEEKYITAEPSVIRRKNRYVCRRCGQSDQSCFAVFWAPSAKRHIT
YCRSCVVMGRADELTSLYSWNQAAENSWEPIKLSWEGTLTDGQKQAADALIEAIKEKQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGSLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKRAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLEKGKLPRSVTDWV
RQKLQSRLPVFLFVPSVHILRKTTDYFQKLNIRAEGVHAEDTCRKDKVKRFREGRVDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEYMNKLAQKNKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=559618 KEF49_RS16965 WP_212099490.1 3341952..3343337(-) (comFA) [Bacillus amyloliquefaciens strain Ba13]
ATGTCCGGAGGTGTTTCAGAATTTTCGGAGGTGCGGGATTTCTTTTACGGCAGGCATTTATTGAGATCTGAGATTCCGTT
TTCTGATCAGTGCATCAAACGTTTTATAGAAGAAAAATATATAACGGCCGAGCCGTCAGTCATCCGCCGGAAAAACCGTT
ATGTCTGCCGTCGATGCGGCCAGTCTGACCAATCCTGCTTTGCGGTATTTTGGGCGCCCTCTGCCAAAAGACATATTACC
TATTGCCGTTCCTGTGTCGTAATGGGAAGGGCTGATGAACTGACGTCATTATACTCTTGGAATCAAGCGGCTGAAAACAG
CTGGGAGCCTATAAAGCTTTCTTGGGAAGGAACACTGACAGATGGACAGAAGCAGGCGGCGGATGCGCTTATAGAAGCGA
TAAAAGAGAAACAAGAGCTGCTGGTATGGGCGGTTTGCGGATCAGGGAAAACAGAGATGCTGTTTCCGGGTATAGAGTTT
GCGCTGAATCACGGCTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTAGAAGTTTCTGCTCTGTACGGCGGCAGTGAAGACAAAGGCAGCTTGACTCCGCTAATGATCTCAA
CCGCTCACCAGCTGATGCGCTATCGAGATATTTTTGATGTAATGATAATAGATGAAGTAGATGCTTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCGGTTGATAAAGCCAGAAAGAAAAACAGCGCCCTAGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAGGGCTGAGGCCGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCGCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTGAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGGCAAAAACTTCAATCACGACTGCCGGTTTTCTTATTCGTTCCGTCTGTACATATATTAAGAAAGACAACGGATTATTT
TCAAAAATTAAACATCCGTGCAGAAGGCGTGCATGCGGAAGACACATGCAGAAAGGATAAAGTGAAACGGTTCAGAGAAG
GACGGGTTGACTTGCTGGTAACGACTACGATTTTAGAAAGAGGCGTTACCGTACCCAAAGTCCAGACTTGTGTCCTCGGC
GCAGAAGCACCTATCTTCACTGAGAGCGCACTCGTACAGATTGCAGGCAGAACGGGCAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGTATGAAGAAAGCAAAAAAACATATAGAATATATGAACAAATTGG
CACAAAAAAATAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(329-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.02

99.132

0.625